Genome structure and molecular phylogeny of the only Eurasian
Boechera species, Boechera falcata (Brassicaceae)

J 2025

Genome structure and molecular phylogeny of the only Eurasian Boechera species, Boechera falcata (Brassicaceae)

ZILOV, Danil; Terezie MALÍK MANDÁKOVÁ; Marina POPOVA; Martin LYSÁK; Michael D WINDHAM et al.

Základní údaje

Originální název

Genome structure and molecular phylogeny of the only Eurasian Boechera species, Boechera falcata (Brassicaceae)

Autoři

ZILOV, Danil; Terezie MALÍK MANDÁKOVÁ; Marina POPOVA; Martin LYSÁK; Michael D WINDHAM a Vladimir BRUKHIN

Vydání

G3-Genes, Genomes, Genetics, Oxford University Press Inc, 2025, 2160-1836

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10602 Biology , Evolutionary biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.200 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00144577

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Boechera falcata; Brassicaceae; genome structure; chromosome-level genome assembly and annotation; chromosome rearrangements; comparative chromosome painting; molecular phylogeny; chloroplast genome

Štítky

14314010

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 3. 2026 17:26, prof. Mgr. Martin Lysák, Ph.D., DSc.

Anotace

V originále

Boechera falcata (Turcz.) Al-Shehbaz, previously known as Arabis turczaninowii Ledeb., is a herbaceous perennial of the East Siberian, boreal-steppe ecotype. It is the sole species of the diverse genus Boechera found on the Eurasian continent, with all other species endemic to North America and Greenland. Likely migrating from North America to Eastern Siberia via the Bering Land Bridge during the Pleistocene glaciation, B. falcata presents a unique case for genomic study. The genus Boechera is notable for its many allodiploid and triploid apomicts, which have arisen through complex hybridization of sexual species and ecotypes. To date, only the genomes of 2 American Boechera species, B. stricta and B. retrofracta, have been sequenced and analyzed. In this study, we sequenced, assembled to the chromosome level, and analyzed the highly homozygous 189.36 Mb genome of B. falcata (2n = 14). Molecular phylogenetic analysis of nuclear and organelle genomes revealed a high degree of relatedness to North American relatives. Cytogenetic analysis identified all 22 genomic blocks of crucifers, showing that 5 of the 7 B. falcata chromosomes are collinear with their ancestral counterparts, while 2 have undergone inversions. Allelic analysis of the apomixis marker APOLLO gene revealed that B. falcata contains only sex alleles. The availability of the B. falcata genome will advance studies of the evolution and phylogeny of Brassicaceae species and the mechanisms of apomixis, providing a crucial resource for future research in plant genetics and breeding.

Návaznosti

GA23-06840S, projekt VaV

Název: Nové evoluční centroméry rostlin

Investor: Grantová agentura ČR, Nové evoluční centroméry rostlin

GA24-11371S, projekt VaV

Název: I rostliny mohou ztratit sexuální touhu: Prozkoumání souvislostí mezi hybridizací, chromozomální reorganizací a evolucí apomixie

Investor: Grantová agentura ČR, I rostliny mohou ztratit sexuální touhu: Prozkoumání souvislostí mezi hybridizací, chromozomální reorganizací a evolucí apomixie

Přiložené soubory

jkaf117.pdf

Citovat

ZILOV, Danil; Terezie MALÍK MANDÁKOVÁ; Marina POPOVA; Martin LYSÁK; Michael D WINDHAM a Vladimir BRUKHIN. Genome structure and molecular phylogeny of the only Eurasian Boechera species, Boechera falcata (Brassicaceae). G3-Genes, Genomes, Genetics. Oxford University Press Inc, 2025, roč. 15, č. 7, s. 1-15. ISSN 2160-1836. Dostupné z: https://doi.org/10.1093/g3journal/jkaf117.

@article{2544199,
   author = {Zilov, Danil and Malík Mandáková, Terezie and Popova, Marina and Lysák, Martin and Windham, Michael D and Brukhin, Vladimir},
   article_number = {7},
   doi = {https://doi.org/10.1093/g3journal/jkaf117},
   keywords = {Boechera falcata; Brassicaceae; genome structure; chromosome-level genome assembly and annotation; chromosome rearrangements; comparative chromosome painting; molecular phylogeny; chloroplast genome},
   language = {eng},
   issn = {2160-1836},
   journal = {G3-Genes, Genomes, Genetics},
   title = {Genome structure and molecular phylogeny of the only Eurasian Boechera species, Boechera falcata (Brassicaceae)},
   url = {https://doi.org/10.1093/g3journal/jkaf117},
   volume = {15},
   year = {2025}
}

TY  - JOUR
ID  - 2544199
AU  - Zilov, Danil - Malík Mandáková, Terezie - Popova, Marina - Lysák, Martin - Windham, Michael D - Brukhin, Vladimir
PY  - 2025
TI  - Genome structure and molecular phylogeny of the only Eurasian Boechera species, Boechera falcata (Brassicaceae)
JF  - G3-Genes, Genomes, Genetics
VL  - 15
IS  - 7
SP  - 1-15
EP  - 1-15
PB  - Oxford University Press Inc
SN  - 21601836
KW  - Boechera falcata
KW  - Brassicaceae
KW  - genome structure
KW  - chromosome-level genome assembly and annotation
KW  - chromosome rearrangements
KW  - comparative chromosome painting
KW  - molecular phylogeny
KW  - chloroplast genome
UR  - https://doi.org/10.1093/g3journal/jkaf117
N2  - Boechera falcata (Turcz.) Al-Shehbaz, previously known as Arabis turczaninowii Ledeb., is a herbaceous perennial of the East Siberian, boreal-steppe ecotype. It is the sole species of the diverse genus Boechera found on the Eurasian continent, with all other species endemic to North America and Greenland. Likely migrating from North America to Eastern Siberia via the Bering Land Bridge during the Pleistocene glaciation, B. falcata presents a unique case for genomic study. The genus Boechera is notable for its many allodiploid and triploid apomicts, which have arisen through complex hybridization of sexual species and ecotypes. To date, only the genomes of 2 American Boechera species, B. stricta and B. retrofracta, have been sequenced and analyzed. In this study, we sequenced, assembled to the chromosome level, and analyzed the highly homozygous 189.36 Mb genome of B. falcata (2n = 14). Molecular phylogenetic analysis of nuclear and organelle genomes revealed a high degree of relatedness to North American relatives. Cytogenetic analysis identified all 22 genomic blocks of crucifers, showing that 5 of the 7 B. falcata chromosomes are collinear with their ancestral counterparts, while 2 have undergone inversions. Allelic analysis of the apomixis marker APOLLO gene revealed that B. falcata contains only sex alleles. The availability of the B. falcata genome will advance studies of the evolution and phylogeny of Brassicaceae species and the mechanisms of apomixis, providing a crucial resource for future research in plant genetics and breeding.
ER  -

ZILOV, Danil; Terezie MALÍK MANDÁKOVÁ; Marina POPOVA; Martin LYSÁK; Michael D WINDHAM a Vladimir BRUKHIN. Genome structure and molecular phylogeny of the only Eurasian Boechera species, Boechera falcata (Brassicaceae). \textit{G3-Genes, Genomes, Genetics}. Oxford University Press Inc, 2025, roč.~15, č.~7, s.~1-15. ISSN~2160-1836. Dostupné z: https://doi.org/10.1093/g3journal/jkaf117.

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