Expanding the CarD interaction network: CrsL is a novel
transcription regulator in actinobacteria

J 2025

Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria

SHOMAN, Mahmoud; Martin ČERNÝ; Jitka JIRAT MATEJCKOVA; Marek SCHWARZ; Nabajyoti BORAH et al.

Základní údaje

Originální název

Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria

Autoři

Vydání

NUCLEIC ACIDS RESEARCH, OXFORD, OXFORD UNIV PRESS, 2025, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 13.100 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00143430

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

CarD; CrsL; Transcription regulation; Actinobacteria; Mycobacterium smegmatis; Mycobacterium tuberculosis; RNA polymerase

Štítky

143160, CF BIC, CF NMR, CF PROT, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 3. 2026 09:33, Mgr. Eva Dubská

Anotace

V originále

Bacterial transcription regulation is critical for adaptation and survival. CarD is an essential transcription factor in mycobacteria involved in the regulation of gene expression. We searched for CarD interaction partners in Mycobacterium smegmatis and identified a novel uncharacterized protein, named CrsL (MSMEG_5890). CrsL is a 5.7 kDa protein shown by NMR to be intrinsically disordered. CrsL homologs are present in actinobacteria, including pathogenic species such as Mycobacterium tuberculosis. CrsL interacts directly with CarD, adopting an ordered structure in the complex, and also binds RNAP, controlling CarD–RNAP association. ChIP-seq showed that CrsL associates with the promoters of actively transcribed genes and ∼75% of these regions are also associated with CarD. RNA-seq revealed ∼50% and ∼66% overlap in differentially expressed genes between CrsL and CarD knockdowns during the exponential and stationary phases, respectively. Among CrsL-regulated genes are DesA desaturase (MSMEG_5773) and DEAD/DEAH-box RNA helicase MSMEG_1930, which contribute to cold stress adaptation. CrsL supports the growth of M. smegmatis at elevated temperature but limits growth in cold environments. In summary, these findings identify CrsL as a novel, conserved CarD-interacting protein playing a key role in mycobacterial stress responses by modulating CarD function.

Návaznosti

EH23_015/0008175, projekt VaV

Název: Inovace České infrastruktury pro integrativní strukturní biologii

GA22-12023S, projekt VaV

Název: Neuspořádanost proteinových struktur vnáší řád do bakteriální transkripce

Investor: Grantová agentura ČR, Neuspořádanost proteinových struktur vnáší řád do bakteriální transkripce

90242, velká výzkumná infrastruktura

Název: CIISB III

Přiložené soubory

2548380.pdf

Citovat

SHOMAN, Mahmoud; Martin ČERNÝ; Jitka JIRAT MATEJCKOVA; Marek SCHWARZ; Nabajyoti BORAH; Viola VANKOVA HAUSNEROVA; Silvia NEVA; Michaela SIKOVA; Hana SANDEROVA; Petr HALADA; Martin HUBALEK; Vera DVORAKOVA; Martin PREVOROVSKY; Jana HOLUBOVA; Ondrej STANEK; Libor KRASNY; Lukáš ŽÍDEK a Jarmila HNILICOVA. Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria. NUCLEIC ACIDS RESEARCH. OXFORD: OXFORD UNIV PRESS, 2025, roč. 53, č. 22, s. 1-24. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkaf1342.

@article{2548380,
   author = {Shoman, Mahmoud and Černý, Martin and Jirat matejckova, Jitka and Schwarz, Marek and Borah, Nabajyoti and Vankova hausnerova, Viola and Neva, Silvia and Sikova, Michaela and Sanderova, Hana and Halada, Petr and Hubalek, Martin and Dvorakova, Vera and Prevorovsky, Martin and Holubova, Jana and Stanek, Ondrej and Krasny, Libor and Žídek, Lukáš and Hnilicova, Jarmila},
   article_location = {OXFORD},
   article_number = {22},
   doi = {https://doi.org/10.1093/nar/gkaf1342},
   keywords = {CarD; CrsL; Transcription regulation; Actinobacteria; Mycobacterium smegmatis; Mycobacterium tuberculosis; RNA polymerase},
   language = {eng},
   issn = {0305-1048},
   journal = {NUCLEIC ACIDS RESEARCH},
   title = {Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria},
   url = {https://academic.oup.com/nar/article/53/22/gkaf1342/8382376},
   volume = {53},
   year = {2025}
}

TY  - JOUR
ID  - 2548380
AU  - Shoman, Mahmoud - Černý, Martin - Jirat matejckova, Jitka - Schwarz, Marek - Borah, Nabajyoti - Vankova hausnerova, Viola - Neva, Silvia - Sikova, Michaela - Sanderova, Hana - Halada, Petr - Hubalek, Martin - Dvorakova, Vera - Prevorovsky, Martin - Holubova, Jana - Stanek, Ondrej - Krasny, Libor - Žídek, Lukáš - Hnilicova, Jarmila
PY  - 2025
TI  - Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria
JF  - NUCLEIC ACIDS RESEARCH
VL  - 53
IS  - 22
SP  - 1-24
EP  - 1-24
PB  - OXFORD UNIV PRESS
SN  - 03051048
KW  - CarD
KW  - CrsL
KW  - Transcription regulation
KW  - Actinobacteria
KW  - Mycobacterium smegmatis
KW  - Mycobacterium tuberculosis
KW  - RNA polymerase
UR  - https://academic.oup.com/nar/article/53/22/gkaf1342/8382376
N2  - Bacterial transcription regulation is critical for adaptation and survival. CarD is an essential transcription factor in mycobacteria involved in the regulation of gene expression. We searched for CarD interaction partners in Mycobacterium smegmatis and identified a novel uncharacterized protein, named CrsL (MSMEG_5890). CrsL is a 5.7 kDa protein shown by NMR to be intrinsically disordered. CrsL homologs are present in actinobacteria, including pathogenic species such as Mycobacterium tuberculosis. CrsL interacts directly with CarD, adopting an ordered structure in the complex, and also binds RNAP, controlling CarD–RNAP association. ChIP-seq showed that CrsL associates with the promoters of actively transcribed genes and ∼75% of these regions are also associated with CarD. RNA-seq revealed ∼50% and ∼66% overlap in differentially expressed genes between CrsL and CarD knockdowns during the exponential and stationary phases, respectively. Among CrsL-regulated genes are DesA desaturase (MSMEG_5773) and DEAD/DEAH-box RNA helicase MSMEG_1930, which contribute to cold stress adaptation. CrsL supports the growth of M. smegmatis at elevated temperature but limits growth in cold environments. In summary, these findings identify CrsL as a novel, conserved CarD-interacting protein playing a key role in mycobacterial stress responses by modulating CarD function.
ER  -

SHOMAN, Mahmoud; Martin ČERNÝ; Jitka JIRAT MATEJCKOVA; Marek SCHWARZ; Nabajyoti BORAH; Viola VANKOVA HAUSNEROVA; Silvia NEVA; Michaela SIKOVA; Hana SANDEROVA; Petr HALADA; Martin HUBALEK; Vera DVORAKOVA; Martin PREVOROVSKY; Jana HOLUBOVA; Ondrej STANEK; Libor KRASNY; Lukáš ŽÍDEK a Jarmila HNILICOVA. Expanding the CarD interaction network: CrsL is a novel transcription regulator in actinobacteria. \textit{NUCLEIC ACIDS RESEARCH}. OXFORD: OXFORD UNIV PRESS, 2025, roč.~53, č.~22, s.~1-24. ISSN~0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkaf1342.

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