Exploring Enterovirus 71 Genome Replication In Situ

a 2025

Exploring Enterovirus 71 Genome Replication In Situ

NEPOVÍMOVÁ, Lucie; Milan EŠNER a Pavel PLEVKA

Základní údaje

Originální název

Exploring Enterovirus 71 Genome Replication In Situ

Autoři

NEPOVÍMOVÁ, Lucie; Milan EŠNER a Pavel PLEVKA

Vydání

Junior Group Workshop for the European Society for Virology: Imaging Viruses, Madrid, Spain, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10607 Virology

Stát vydavatele

Španělsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00143708

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

enterovirus; electron microscopy; cryo-electron tomography; replication dynamics; viral assembly; fluorescent visualisation

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 2. 2026 04:11, Mgr. Eva Dubská

Anotace

V originále

Background and Aims. Enterovirus 71 (EV71), a Picornaviridae family member, is a major cause of hand-foot-and-mouth disease, predominantly affecting young children. Severe cases can result in neurological complications with mortality rates reaching 19%. Escalating outbreaks in the Asia-Pacific region highlight the urgent need for novel therapeutic approaches. Current vaccines target specific serotypes but lack evidence of cross-protection, and antiviral therapies remain unavailable due to limited knowledge of EV71's replication cycle. This study aims to investigate unexplored aspects of EV71 RNA replication, including its coupling with genome packaging and viral assembly. Methods. We developed a fluorescent reporter system to specifically label EV71 replication sites while preserving the natural viral genome and replication process. The reporter serves as a key tool for advanced imaging techniques, including correlative light and electron microscopy and cryo-electron tomography, to visualise replication dynamics and viral assembly at molecular level. Results. Preliminary results demonstrate successful cloning of the fluorescent reporter system. Functional validation is underway using fluorescence microscopy, providing insights into the reporter’s potential to track replication in both live and fixed cells.

Návaznosti

EH22_008/0004607, projekt VaV

Název: Nové technologie pro translační výzkum ve farmaceutických vědách /NETPHARM

Přiložené soubory

Nepovimova_jsnhcdup.pdf

Nepovimova.pdf

Citovat

NEPOVÍMOVÁ, Lucie; Milan EŠNER a Pavel PLEVKA. Exploring Enterovirus 71 Genome Replication In Situ. In Junior Group Workshop for the European Society for Virology: Imaging Viruses, Madrid, Spain. 2025.

@proceedings{2554537,
   author = {Nepovímová, Lucie and Ešner, Milan and Plevka, Pavel},
   booktitle = {Junior Group Workshop for the European Society for Virology: Imaging Viruses, Madrid, Spain},
   keywords = {enterovirus; electron microscopy; cryo-electron tomography; replication dynamics; viral assembly; fluorescent visualisation},
   language = {eng},
   title = {Exploring Enterovirus 71 Genome Replication In Situ},
   url = {https://www.cbm.uam.es/index.php/events/junior-group-workshop-for-the-european-society-for-virology-imaging-viruses/?utm_source=chatgpt.com},
   year = {2025}
}

TY  - CONF
ID  - 2554537
AU  - Nepovímová, Lucie - Ešner, Milan - Plevka, Pavel
PY  - 2025
TI  - Exploring Enterovirus 71 Genome Replication In Situ
KW  - enterovirus
KW  - electron microscopy
KW  - cryo-electron tomography
KW  - replication dynamics
KW  - viral assembly
KW  - fluorescent visualisation
UR  - https://www.cbm.uam.es/index.php/events/junior-group-workshop-for-the-european-society-for-virology-imaging-viruses/?utm_source=chatgpt.com
N2  - Background and Aims. Enterovirus 71 (EV71), a Picornaviridae family member, is a major cause of hand-foot-and-mouth disease, predominantly affecting young children. Severe cases can result in neurological complications with mortality rates reaching 19%. Escalating outbreaks in the Asia-Pacific region highlight the urgent need for novel therapeutic approaches. Current vaccines target specific serotypes but lack evidence of cross-protection, and antiviral therapies remain unavailable due to limited knowledge of EV71's replication cycle. This study aims to investigate unexplored aspects of EV71 RNA replication, including its coupling with genome packaging and viral assembly. Methods. We developed a fluorescent reporter system to specifically label EV71 replication sites while preserving the natural viral genome and replication process. The reporter serves as a key tool for advanced imaging techniques, including correlative light and electron microscopy and cryo-electron tomography, to visualise replication dynamics and viral assembly at molecular level. Results. Preliminary results demonstrate successful cloning of the fluorescent reporter system. Functional validation is underway using fluorescence microscopy, providing insights into the reporter’s potential to track replication in both live and fixed cells.
ER  -

NEPOVÍMOVÁ, Lucie; Milan EŠNER a Pavel PLEVKA. Exploring Enterovirus 71 Genome Replication In Situ. In \textit{Junior Group Workshop for the European Society for Virology: Imaging Viruses, Madrid, Spain}. 2025.

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