CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH

a 2025

CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH

SZMITKOWSKA, Agnieszka; Lucia TOMOVIČOVÁ; Amel YAMOUNE; Thomas DEPAEPE; Blanka PEKÁROVÁ et al.

Základní údaje

Originální název

CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH

Autoři

SZMITKOWSKA, Agnieszka; Lucia TOMOVIČOVÁ ; Amel YAMOUNE; Thomas DEPAEPE; Blanka PEKÁROVÁ ; Abigail Rubiato CUYACOT; Markéta ŽĎÁRSKÁ; Josef HOUSER; Jan KOMÁREK ; Kenneth Wayne BERENDZEN; Virtudes MIRA-RODADO; Paul TARR; Lukáš ŽÍDEK; Michaela WIMMEROVÁ; Klaus HARTER; Elliot MEYEROWITZ; Dominique Van Der STRAETEN a Jan HEJÁTKO

Vydání

Plan Biology CS 2025, Bratislava, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10611 Plant sciences, botany

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00143778

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

biosynthesis; cytokinins; ethylene; multistep phosphorelay; signaling

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2026 09:22, Mgr. Eva Dubská

Anotace

V originále

The interconnected action of cytokinins and ethylene in the control of plant growth was demonstrated. However, the extent of the crosstalk and the underlying molecular mechanisms remained mostly elusive. We and others’ have demonstrated that multistep phosphorelay (MSP) pathway, previously thought mainly to mediate cytokinin signaling, is under control of ethylene through the histidine kinase (HK) activity of ethylene sensor ETR1. Here we show that although ETR1 is an active HK, its receiver domain (ETR1RD) is structurally and functionally unable to accept the phosphate from the phosphorylated His in the ETR1 HK domain (ETR1HK) to initiate the phosphorelay to AHPs, the next downstream link in MSP signaling. Instead, ETR1 interacts with another HK AHK5 and transfers the phosphate from ETR1HK through the receiver domain of AHK5 (AHK5RD), and subsequently to AHP1, AHP2 and AHP3, independently of the HK activity of AHK5. We show that AHK5 is necessary for ethylene-initiated, but not cytokinin-initiated, MSP signaling in planta and is involved in the hormonal control of root growth and architecture in Arabidopsis. Furthermore, we have identified novel mechanism of transcriptional regulation based on the interaction of members of ethylene and MSP signaling pathways in the spatial-specific control of cytokinin-induced ethylene biosynthesis, mediating the cytokinin-induced, ethylene- regulated root growth. We propose that the aforementioned regulatory network represents a molecular basis for the existence of previously proposed morphogenic field combining the properties of both cytokinins and ethylene, controlling, together with auxin, root growth and patterning.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

LUAUS24277, projekt VaV

Název: Objasnění mechanismů hormonálních regulací longitudinální zonace kořene

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Objasnění mechanismů hormonálních regulací longitudinální zonace kořene, INTER-ACTION (USA)

Přiložené soubory

PB_CS_2025___Student_Days_Book_of_Abstract.pdf

Citovat

SZMITKOWSKA, Agnieszka; Lucia TOMOVIČOVÁ; Amel YAMOUNE; Thomas DEPAEPE; Blanka PEKÁROVÁ; Abigail Rubiato CUYACOT; Markéta ŽĎÁRSKÁ; Josef HOUSER; Jan KOMÁREK; Kenneth Wayne BERENDZEN; Virtudes MIRA-RODADO; Paul TARR; Lukáš ŽÍDEK; Michaela WIMMEROVÁ; Klaus HARTER; Elliot MEYEROWITZ; Dominique Van Der STRAETEN a Jan HEJÁTKO. CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH. In Plan Biology CS 2025, Bratislava. 2025.

@proceedings{2556820,
   author = {Szmitkowska, Agnieszka and Tomovičová, Lucia and Yamoune, Amel and Depaepe, Thomas and Pekárová, Blanka and Cuyacot, Abigail Rubiato and Žďárská, Markéta and Houser, Josef and Komárek, Jan and Berendzen, Kenneth Wayne and MiraandRodado, Virtudes and Tarr, Paul and Žídek, Lukáš and Wimmerová, Michaela and Harter, Klaus and Meyerowitz, Elliot and Straeten, Dominique Van Der and Hejátko, Jan},
   booktitle = {Plan Biology CS 2025, Bratislava},
   keywords = {biosynthesis; cytokinins; ethylene; multistep phosphorelay; signaling},
   language = {eng},
   title = {CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH},
   url = {https://csebr.cz/plant-biology-cs-2025/},
   year = {2025}
}

TY  - CONF
ID  - 2556820
AU  - Szmitkowska, Agnieszka - Tomovičová, Lucia - Yamoune, Amel - Depaepe, Thomas - Pekárová, Blanka - Cuyacot, Abigail Rubiato - Žďárská, Markéta - Houser, Josef - Komárek, Jan - Berendzen, Kenneth Wayne - Mira-Rodado, Virtudes - Tarr, Paul - Žídek, Lukáš - Wimmerová, Michaela - Harter, Klaus - Meyerowitz, Elliot - Straeten, Dominique Van Der - Hejátko, Jan
PY  - 2025
TI  - CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH
KW  - biosynthesis
KW  - cytokinins
KW  - ethylene
KW  - multistep phosphorelay
KW  - signaling
UR  - https://csebr.cz/plant-biology-cs-2025/
N2  - The interconnected action of cytokinins and ethylene in the control of plant growth was demonstrated. However, the extent of the crosstalk and the underlying molecular mechanisms remained mostly elusive. We and others’ have demonstrated that multistep phosphorelay (MSP) pathway, previously thought mainly to mediate cytokinin signaling, is under control of ethylene through the histidine kinase (HK) activity of ethylene sensor ETR1. Here we show that although ETR1 is an active HK, its receiver domain (ETR1RD) is structurally and functionally unable to accept the phosphate from the phosphorylated His in the ETR1 HK domain (ETR1HK) to initiate the phosphorelay to AHPs, the next downstream link in MSP signaling. Instead, ETR1 interacts with another HK AHK5 and transfers the phosphate from ETR1HK through the receiver domain of AHK5 (AHK5RD), and subsequently to AHP1, AHP2 and AHP3, independently of the HK activity of AHK5. We show that AHK5 is necessary for ethylene-initiated, but not cytokinin-initiated, MSP signaling in planta and is involved in the hormonal control of root growth and architecture in Arabidopsis. Furthermore, we have identified novel mechanism of transcriptional regulation based on the interaction of members of ethylene and MSP signaling pathways in the spatial-specific control of cytokinin-induced ethylene biosynthesis, mediating the cytokinin-induced, ethylene- regulated root growth. We propose that the aforementioned regulatory network represents a molecular basis for the existence of previously proposed morphogenic field combining the properties of both cytokinins and ethylene, controlling, together with auxin, root growth and patterning.
ER  -

SZMITKOWSKA, Agnieszka; Lucia TOMOVIČOVÁ; Amel YAMOUNE; Thomas DEPAEPE; Blanka PEKÁROVÁ; Abigail Rubiato CUYACOT; Markéta ŽĎÁRSKÁ; Josef HOUSER; Jan KOMÁREK; Kenneth Wayne BERENDZEN; Virtudes MIRA-RODADO; Paul TARR; Lukáš ŽÍDEK; Michaela WIMMEROVÁ; Klaus HARTER; Elliot MEYEROWITZ; Dominique Van Der STRAETEN a Jan HEJÁTKO. CYTOKININ/ETHYLENE CROSSTALK IN THE CONTROL OF ROOT GROWTH. In \textit{Plan Biology CS 2025, Bratislava}. 2025.

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