How do transcription and alternative splicing shape the seed
heat-stress response?

a 2025

How do transcription and alternative splicing shape the seed heat-stress response?

GUENNICH, Oussama

Základní údaje

Originální název

How do transcription and alternative splicing shape the seed heat-stress response?

Autoři

GUENNICH, Oussama

Vydání

TANGENC Conference 2025, Brno, 2025

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10611 Plant sciences, botany

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/25:00143980

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Heat stress response (HSR) Seed development Transcriptional regulation Alternative splicing Proteo-transcriptomics

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 16. 3. 2026 10:59, Mgr. Eva Dubská

Anotace

V originále

High temperature negatively affects plant reproduction and seed yield, posing a challenge for global food security. To better understand the molecular mechanisms underlying the seed heat shock response (HSR), we applied an integrated proteo-transcriptomic approach to analyze gene expression and protein abundance during seed development under long-term heat stress. Our results indicate that transcriptional regulation represents the main driver of the HSR. A positive correlation between transcript and protein abundance suggests that transcriptional upregulation is frequently reflected at the proteome level. Promoter analysis revealed that heat shock transcription factors HSFA1abd and HSFA2 likely regulate a large proportion of heat-induced genes. Binding sites for these factors were detected upstream of most upregulated genes at early stages of seed development. In parallel, we analyzed the contribution of alternative splicing (AS) to the heat stress response. Although widespread AS changes occur during heat stress, their impact on overall mRNA and protein abundance appears limited. Nevertheless, proteomic analyses confirmed the presence of non-canonical protein isoforms generated by alternative splicing, indicating that AS contributes to proteome diversity during the seed heat stress response.

Návaznosti

EH22_008/0004581, projekt VaV

Název: Nové poznatky pro plodiny nové generace

Přiložené soubory

10_TALK_24.11.25_Guennich_TANGENC.pptx

Citovat

GUENNICH, Oussama. How do transcription and alternative splicing shape the seed heat-stress response? In TANGENC Conference 2025, Brno. 2025.

@proceedings{2560924,
   author = {Guennich, Oussama},
   booktitle = {TANGENC Conference 2025, Brno},
   keywords = {Heat stress response (HSR) Seed development Transcriptional regulation Alternative splicing Proteo-transcriptomics},
   language = {eng},
   title = {How do transcription and alternative splicing shape the seed heat-stress response?},
   url = {https://www.ceitec.eu/tangenc-conference-2025/a5272},
   year = {2025}
}

TY  - CONF
ID  - 2560924
AU  - Guennich, Oussama
PY  - 2025
TI  - How do transcription and alternative splicing shape the seed heat-stress response?
KW  - Heat stress response (HSR) Seed development Transcriptional regulation Alternative splicing Proteo-transcriptomics
UR  - https://www.ceitec.eu/tangenc-conference-2025/a5272
N2  - High temperature negatively affects plant reproduction and seed yield, posing a challenge for global food security. To better understand the molecular mechanisms underlying the seed heat shock response (HSR), we applied an integrated proteo-transcriptomic approach to analyze gene expression and protein abundance during seed development under long-term heat stress. Our results indicate that transcriptional regulation represents the main driver of the HSR. A positive correlation between transcript and protein abundance suggests that transcriptional upregulation is frequently reflected at the proteome level. Promoter analysis revealed that heat shock transcription factors HSFA1abd and HSFA2 likely regulate a large proportion of heat-induced genes. Binding sites for these factors were detected upstream of most upregulated genes at early stages of seed development. In parallel, we analyzed the contribution of alternative splicing (AS) to the heat stress response. Although widespread AS changes occur during heat stress, their impact on overall mRNA and protein abundance appears limited. Nevertheless, proteomic analyses confirmed the presence of non-canonical protein isoforms generated by alternative splicing, indicating that AS contributes to proteome diversity during the seed heat stress response.
ER  -

GUENNICH, Oussama. How do transcription and alternative splicing shape the seed heat-stress response? In \textit{TANGENC Conference 2025, Brno}. 2025.

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