The combination of mupirocin and Kayvirus broadens the
decolonization effect against Staphylococcus aureus

J 2026

The combination of mupirocin and Kayvirus broadens the decolonization effect against Staphylococcus aureus

SIVÁKOVÁ, Alena; Eliška FIGALLOVÁ; Tibor BOTKA; Lukáš VACEK; Jan TKADLEC et al.

Základní údaje

Originální název

The combination of mupirocin and Kayvirus broadens the decolonization effect against Staphylococcus aureus

Autoři

Vydání

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, USA, SPRINGER, 2026, 0175-7598

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 4.300 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Staphylococcus aureus; Panton-Valentine leukocidin; Decolonization; Mupirocin; Phage therapy; Kayvirus

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 6. 2026 14:24, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

Staphylococcus aureus strains cause a wide range of infections in humans, often with the potential for complications such as surgical site infections. The production of Panton-Valentin leukocidin (PVL) by certain strains of S. aureus is clinically associated with chronic or recurrent infections, which typically require decolonization, most often with mupirocin. As increased mupirocin use promotes the emergence of resistance, this study investigated the coadministration of mupirocin and therapeutic Kayvirus bacteriophage as a potential strategy to enhance treatment efficacy and prevent the development of new resistance. We collected and evaluated 37 PVL-encoding S. aureus strains from wound samples. Among these, 22% were methicillin-resistant, and 11% were resistant to the tested phage, but all were susceptible to mupirocin. To assess interactions between mupirocin and the phage in PVL-positive strains with varying levels of mupirocin resistance, we used lysogenization by PVL-encoding phage and adaptive laboratory evolution of clinical strains. In mupirocin-susceptible strains, lytic phage efficacy decreased due to altered protein synthesis caused by the interaction of mupirocin with isoleucyl-tRNA synthetase, whereas mupirocin efficacy was unaffected. In contrast, the advantage of combined administration was observed in mupirocin-resistant strains susceptible to phages, as their altered or alternative synthetase allowed protein synthesis to continue, enabling phage proliferation and bacterial lysis, even in the presence of mupirocin. This in vitro study demonstrates that mupirocin in combination with Kayvirus broadens the spectrum of strains susceptible to treatment and that the phage used prevents the development of mupirocin resistance.

Návaznosti

MUNI/A/1759/2025, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární a buněčné biologie a genetiky 14

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární a buněčné biologie a genetiky 14

NU21J-05-00035, projekt VaV

Název: Synergie lytických bakteriofágů a antibiotik v léčbě povrchových infekcí způsobených Staphylococcus aureus (Akronym: PAS-based therapy of S. aureus infections)

Investor: Ministerstvo zdravotnictví ČR, Synergy of lytic bacteriophages and antibiotics in the therapy of topical infections of Staphylococcus aureus, Podprogram 2 - juniorský - výzkumníci do 35 let

Přiložené soubory

s00253-026-13814-0.pdf

Citovat

SIVÁKOVÁ, Alena; Eliška FIGALLOVÁ; Tibor BOTKA; Lukáš VACEK; Jan TKADLEC; Jan VRBSKÝ; Martin OSOWSKI; Petr PETRÁŠ; Dominika POLAŠTIK KLEKNEROVÁ; Milada DVOŘÁČKOVÁ; Pavlína URBANOVÁ; Roman PANTŮČEK a Filip RŮŽIČKA. The combination of mupirocin and Kayvirus broadens the decolonization effect against Staphylococcus aureus. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. USA: SPRINGER, 2026, roč. 110, č. 1, s. 157-172. ISSN 0175-7598. Dostupné z: https://doi.org/10.1007/s00253-026-13814-0.

@article{2573179,
   author = {Siváková, Alena and Figallová, Eliška and Botka, Tibor and Vacek, Lukáš and Tkadlec, Jan and Vrbský, Jan and Osowski, Martin and Petráš, Petr and Polaštik Kleknerová, Dominika and Dvořáčková, Milada and Urbanová, Pavlína and Pantůček, Roman and Růžička, Filip},
   article_location = {USA},
   article_number = {1},
   doi = {https://doi.org/10.1007/s00253-026-13814-0},
   keywords = {Staphylococcus aureus; Panton-Valentine leukocidin; Decolonization; Mupirocin; Phage therapy; Kayvirus},
   language = {eng},
   issn = {0175-7598},
   journal = {APPLIED MICROBIOLOGY AND BIOTECHNOLOGY},
   title = {The combination of mupirocin and Kayvirus broadens the decolonization effect against Staphylococcus aureus},
   url = {https://link.springer.com/article/10.1007/s00253-026-13814-0},
   volume = {110},
   year = {2026}
}

TY  - JOUR
ID  - 2573179
AU  - Siváková, Alena - Figallová, Eliška - Botka, Tibor - Vacek, Lukáš - Tkadlec, Jan - Vrbský, Jan - Osowski, Martin - Petráš, Petr - Polaštik Kleknerová, Dominika - Dvořáčková, Milada - Urbanová, Pavlína - Pantůček, Roman - Růžička, Filip
PY  - 2026
TI  - The combination of mupirocin and Kayvirus broadens the decolonization effect against Staphylococcus aureus
JF  - APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
VL  - 110
IS  - 1
SP  - 157
EP  - 157
PB  - SPRINGER
SN  - 01757598
KW  - Staphylococcus aureus
KW  - Panton-Valentine leukocidin
KW  - Decolonization
KW  - Mupirocin
KW  - Phage therapy
KW  - Kayvirus
UR  - https://link.springer.com/article/10.1007/s00253-026-13814-0
N2  - Staphylococcus aureus strains cause a wide range of infections in humans, often with the potential for complications such as surgical site infections. The production of Panton-Valentin leukocidin (PVL) by certain strains of S. aureus is clinically associated with chronic or recurrent infections, which typically require decolonization, most often with mupirocin. As increased mupirocin use promotes the emergence of resistance, this study investigated the coadministration of mupirocin and therapeutic Kayvirus bacteriophage as a potential strategy to enhance treatment efficacy and prevent the development of new resistance. We collected and evaluated 37 PVL-encoding S. aureus strains from wound samples. Among these, 22% were methicillin-resistant, and 11% were resistant to the tested phage, but all were susceptible to mupirocin. To assess interactions between mupirocin and the phage in PVL-positive strains with varying levels of mupirocin resistance, we used lysogenization by PVL-encoding phage and adaptive laboratory evolution of clinical strains. In mupirocin-susceptible strains, lytic phage efficacy decreased due to altered protein synthesis caused by the interaction of mupirocin with isoleucyl-tRNA synthetase, whereas mupirocin efficacy was unaffected. In contrast, the advantage of combined administration was observed in mupirocin-resistant strains susceptible to phages, as their altered or alternative synthetase allowed protein synthesis to continue, enabling phage proliferation and bacterial lysis, even in the presence of mupirocin. This in vitro study demonstrates that mupirocin in combination with Kayvirus broadens the spectrum of strains susceptible to treatment and that the phage used prevents the development of mupirocin resistance.
ER  -

SIVÁKOVÁ, Alena; Eliška FIGALLOVÁ; Tibor BOTKA; Lukáš VACEK; Jan TKADLEC; Jan VRBSKÝ; Martin OSOWSKI; Petr PETRÁŠ; Dominika POLAŠTIK KLEKNEROVÁ; Milada DVOŘÁČKOVÁ; Pavlína URBANOVÁ; Roman PANTŮČEK a Filip RŮŽIČKA. The combination of mupirocin and Kayvirus broadens the decolonization effect against Staphylococcus aureus. \textit{APPLIED MICROBIOLOGY AND BIOTECHNOLOGY}. USA: SPRINGER, 2026, roč.~110, č.~1, s.~157-172. ISSN~0175-7598. Dostupné z: https://doi.org/10.1007/s00253-026-13814-0.

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