Physiological and genomic characterization of
Methanothermobacter thermautotrophicus strain Z-73A, an isolate
from deep underground

J 2026

Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground

HANIŠÁKOVÁ, Nikola; Anna KOHOUTOVÁ; Eva KOTRLOVÁ; Tijana JOKSIĆ; Haizea Juncal AIXELÁ VÁZQUEZ et al.

Základní údaje

Originální název

Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground

Autoři

Vydání

BIORESOURCE TECHNOLOGY, ELSEVIER SCI LTD, 2026, 0960-8524

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 9.000 v roce 2024

Označené pro přenos do RIV

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1016/j.biortech.2026.135519

Klíčová slova anglicky

Methane; CO2 capture; Biomethanation; Nanosilver; Anaerobic corrosion; Methanogens; Archaea

Štítky

14314010, 14315010

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 8. 2026 12:22, Mgr. Tibor Botka, Ph.D.

Anotace

V originále

Underground biomethanation represents a promising solution for green energy storage. An isolate of Methanothermobacter thermautotrophicus was obtained in previous study from an underground gas reservoir where it played a key role in a successful in-situ biomethanation experiment. In this study, the physiological and environmental tolerances of the strain are characterised. Optimal methane evolution rates were observed at 55–65 °C and pH 6.5–7.0, methane production was significantly increased by agitation (up to 2.29 mmol CH4·h−1·L−1). The strain showed higher tolerance to NH4Cl (up to 0.6 M) than to NaCl (optimal 0.04–0.4 M). The strain was also tested for its resistance to silver nanoparticles. The whole-genome sequence of the strain was determined, and the corrosion potential was evaluated. To visualise the cells, Scanning Electron Microscopy (SEM) and a method of Advanced Environmental Scanning Electron Microscopy (A-ESEM) were used. These findings describe the behaviour of M. thermautotrophicus under suboptimal conditions and highlight the need for further optimization to improve methane production yields.

Návaznosti

MUNI/A/1774/2024, interní kód MU

Název: Podpora výzkumné činnosti studentů Mikrobiologie 5

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů Mikrobiologie 5

Přiložené soubory

2584997.pdf

Požádat o autorskou verzi souboru

Citovat

HANIŠÁKOVÁ, Nikola; Anna KOHOUTOVÁ; Eva KOTRLOVÁ; Tijana JOKSIĆ; Haizea Juncal AIXELÁ VÁZQUEZ; Tomáš VÍTĚZ; Tibor BOTKA; Josef ZEMAN; Vlasta ŠTĚPÁNOVÁ; Vilém NEDĚLA; Markéta LASKAFELDOVÁ; Jakub JAVŮREK a Monika VÍTĚZOVÁ. Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground. BIORESOURCE TECHNOLOGY. ELSEVIER SCI LTD, 2026, roč. 462, December, s. 135519-135531. ISSN 0960-8524. Dostupné z: https://doi.org/10.1016/j.biortech.2026.135519.

@article{2584997,
   author = {Hanišáková, Nikola and Kohoutová, Anna and Kotrlová, Eva and Joksić, Tijana and Aixelá Vázquez, Haizea Juncal and Vítěz, Tomáš and Botka, Tibor and Zeman, Josef and Štěpánová, Vlasta and Neděla, Vilém and Laskafeldová, Markéta and Javůrek, Jakub and Vítězová, Monika},
   article_number = {December},
   doi = {https://doi.org/10.1016/j.biortech.2026.135519},
   keywords = {Methane; CO2 capture; Biomethanation; Nanosilver; Anaerobic corrosion; Methanogens; Archaea},
   language = {eng},
   issn = {0960-8524},
   journal = {BIORESOURCE TECHNOLOGY},
   title = {Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground},
   url = {https://doi.org/10.1016/j.biortech.2026.135519},
   volume = {462},
   year = {2026}
}

TY  - JOUR
ID  - 2584997
AU  - Hanišáková, Nikola - Kohoutová, Anna - Kotrlová, Eva - Joksić, Tijana - Aixelá Vázquez, Haizea Juncal - Vítěz, Tomáš - Botka, Tibor - Zeman, Josef - Štěpánová, Vlasta - Neděla, Vilém - Laskafeldová, Markéta - Javůrek, Jakub - Vítězová, Monika
PY  - 2026
TI  - Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground
JF  - BIORESOURCE TECHNOLOGY
VL  - 462
IS  - December
SP  - 135519
EP  - 135519
PB  - ELSEVIER SCI LTD
SN  - 09608524
KW  - Methane
KW  - CO2 capture
KW  - Biomethanation
KW  - Nanosilver
KW  - Anaerobic corrosion
KW  - Methanogens
KW  - Archaea
UR  - https://doi.org/10.1016/j.biortech.2026.135519
N2  - Underground biomethanation represents a promising solution for green energy storage. An isolate of Methanothermobacter thermautotrophicus was obtained in previous study from an underground gas reservoir where it played a key role in a successful in-situ biomethanation experiment. In this study, the physiological and environmental tolerances of the strain are characterised. Optimal methane evolution rates were observed at 55–65 °C and pH 6.5–7.0, methane production was significantly increased by agitation (up to 2.29 mmol CH4·h−1·L−1). The strain showed higher tolerance to NH4Cl (up to 0.6 M) than to NaCl (optimal 0.04–0.4 M). The strain was also tested for its resistance to silver nanoparticles. The whole-genome sequence of the strain was determined, and the corrosion potential was evaluated. To visualise the cells, Scanning Electron Microscopy (SEM) and a method of Advanced Environmental Scanning Electron Microscopy (A-ESEM) were used. These findings describe the behaviour of M. thermautotrophicus under suboptimal conditions and highlight the need for further optimization to improve methane production yields.
ER  -

HANIŠÁKOVÁ, Nikola; Anna KOHOUTOVÁ; Eva KOTRLOVÁ; Tijana JOKSI$\backslash$'C; Haizea Juncal AIXELÁ VÁZQUEZ; Tomáš VÍTĚZ; Tibor BOTKA; Josef ZEMAN; Vlasta ŠTĚPÁNOVÁ; Vilém NEDĚLA; Markéta LASKAFELDOVÁ; Jakub JAVŮREK a Monika VÍTĚZOVÁ. Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground. \textit{BIORESOURCE TECHNOLOGY}. ELSEVIER SCI LTD, 2026, roč.~462, December, s.~135519-135531. ISSN~0960-8524. Dostupné z: https://doi.org/10.1016/j.biortech.2026.135519.

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