TAS49-a dispersed repetitive sequence isolated from
subtelomeric regions of Nicotiana tomentosiformis chromosomes

J 1999

TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes

HORÁKOVÁ, Mirka a Jiří FAJKUS

Základní údaje

Originální název

TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes

Autoři

HORÁKOVÁ, Mirka (203 Česká republika) a Jiří FAJKUS (203 Česká republika, garant)

Vydání

Genome, Canada, NRC Canada, 1999, 0831-2796

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Kanada

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.847

Kód RIV

RIV/00216224:14310/99:00002246

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

repetitive DNA sequence; subtelomere; plant; Nicotiana

Štítky

Nicotiana, plant, repetitive DNA sequence, subtelomere

Změněno: 25. 6. 2008 08:28, prof. RNDr. Jiří Fajkus, CSc.

Anotace

V originále

We have isolated and characterized a new repetitive sequence, TAS49, from terminal restriction fragments of Nicotiana tomentosiformis genomic DNA by means of a modified vectorette approach. The TAS49 was found directly attached to telomeres of N. tabacum and one of its ancestors, N. tomentosiformis, and also at inner chromosome locations. No association with telomeres was detected neither in N. otophora nor in the second tobacco ancestor, N. sylvestris. PCR and Southern hybridization reveal similarities in the arrangement of TAS49 on the chromosomes of 9 species of the genus Nicotiana, implying its occurrence as a subunit of a conserved complex DNA repeat. TAS49 belongs to the family of dispersed repetitive sequences without features of transposons. The copy number of TAS49 varies widely in the genomes of 8 species analyzed being lowest in N. sylvestris, with 3300 copies per diploid genome. In N. tomentosiformis, TAS49 forms about 0.56% of the diploid genome, corresponding to 17400 copies. TAS49 units are about 460 bp long and show about 90% of mutual homology, but no significant homology to DNA sequences deposited in GenBank and EMBL. Although genomic clones of TAS49 contain an open reading frame encoding a proline-rich protein similar to plant extensins, no mRNA transcript was detected. TAS49 is extensively methylated at CpG and CpNpG sites and its chromatin forms nucleosomes phased with a 170 +/- 8 bp periodicity.

Návaznosti

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

VS97032, projekt VaV

Název: Analýza biologicky významných molekulárních komplexů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Analýza biologicky významných molekulárních komplexů

Citovat

HORÁKOVÁ, Mirka a Jiří FAJKUS. TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes. Genome. Canada: NRC Canada, 1999, roč. 43, č. 2, s. 273-283. ISSN 0831-2796.

@article{322211,
   author = {Horáková, Mirka and Fajkus, Jiří},
   article_location = {Canada},
   article_number = {2},
   keywords = {repetitive DNA sequence; subtelomere; plant; Nicotiana},
   language = {eng},
   issn = {0831-2796},
   journal = {Genome},
   title = {TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes},
   volume = {43},
   year = {1999}
}

TY  - JOUR
ID  - 322211
AU  - Horáková, Mirka - Fajkus, Jiří
PY  - 1999
TI  - TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes
JF  - Genome
VL  - 43
IS  - 2
SP  - 273
EP  - 273
PB  - NRC Canada
SN  - 08312796
KW  - repetitive DNA sequence
KW  - subtelomere
KW  - plant
KW  - Nicotiana
N2  - We have isolated and characterized a new repetitive sequence, TAS49, from terminal restriction fragments of Nicotiana tomentosiformis genomic DNA by means of a modified vectorette approach. The TAS49 was found directly attached to telomeres of N. tabacum and one of its ancestors, N. tomentosiformis, and also at inner chromosome locations. No association with telomeres was detected neither in N. otophora nor in the second tobacco ancestor, N. sylvestris. PCR and Southern hybridization reveal similarities in the arrangement of TAS49 on the chromosomes of 9 species of the genus Nicotiana, implying its occurrence as a subunit of a conserved complex DNA repeat. TAS49 belongs to the family of dispersed repetitive sequences without features of transposons. The copy number of TAS49 varies widely in the genomes of 8 species analyzed being lowest in N. sylvestris, with 3300 copies per diploid genome. In N. tomentosiformis, TAS49 forms about 0.56% of the diploid genome, corresponding to 17400 copies. TAS49 units are about 460 bp long and show about 90% of mutual homology, but no significant homology to DNA sequences deposited in GenBank and EMBL. Although genomic clones of TAS49 contain an open reading frame encoding a proline-rich protein similar to plant extensins, no mRNA transcript was detected. TAS49 is extensively methylated at CpG and CpNpG sites and its chromatin forms nucleosomes phased with a 170 +/- 8 bp periodicity.
ER  -

HORÁKOVÁ, Mirka a Jiří FAJKUS. TAS49-a dispersed repetitive sequence isolated from subtelomeric regions of Nicotiana tomentosiformis chromosomes. \textit{Genome}. Canada: NRC Canada, 1999, roč.~43, č.~2, s.~273-283. ISSN~0831-2796.

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