Application of Elimination Voltammetry to Adsorptive Stripping
of DNA

J 2000

Application of Elimination Voltammetry to Adsorptive Stripping of DNA

TRNKOVÁ, Libuše, René KIZEK a Oldřich DRAČKA

Základní údaje

Originální název

Application of Elimination Voltammetry to Adsorptive Stripping of DNA

Autoři

TRNKOVÁ, Libuše, René KIZEK a Oldřich DRAČKA

Vydání

Electroanalysis, Weinheim, Wiley-VCH Verlag GmbH, 2000, 1040-0397

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10405 Electrochemistry

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.972

Kód RIV

RIV/00216224:14310/00:00003066

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000088963200006

Klíčová slova anglicky

Linear scan voltametry; Elimination voltammetry; Irreversible current; Adsorptive Stripping Voltammetry; DNA

Štítky

adsorptive stripping voltammetry, DNA, Elimination voltammetry, Irreversible current, Linear scan voltametry

Změněno: 12. 3. 2001 16:45, prof. RNDr. Libuše Trnková, CSc.

Anotace

V originále

Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak (due to the reduction of adenine and cytosine residues), the elimination provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter.

Návaznosti

GV204/97/K084, projekt VaV

Název: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Grantová agentura ČR, Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

MSM 143100011, záměr

Název: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Citovat

TRNKOVÁ, Libuše, René KIZEK a Oldřich DRAČKA. Application of Elimination Voltammetry to Adsorptive Stripping of DNA. Electroanalysis. Weinheim: Wiley-VCH Verlag GmbH, 2000, roč. 2000, č. 12, s. 905-911. ISSN 1040-0397.

@article{325653,
   author = {Trnková, Libuše and Kizek, René and Dračka, Oldřich},
   article_location = {Weinheim},
   article_number = {12},
   keywords = {Linear scan voltametry; Elimination voltammetry; Irreversible current; Adsorptive Stripping Voltammetry; DNA},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Application of Elimination Voltammetry to Adsorptive Stripping of DNA},
   volume = {2000},
   year = {2000}
}

TY  - JOUR
ID  - 325653
AU  - Trnková, Libuše - Kizek, René - Dračka, Oldřich
PY  - 2000
TI  - Application of Elimination Voltammetry to Adsorptive Stripping of DNA
JF  - Electroanalysis
VL  - 2000
IS  - 12
SP  - 905
EP  - 905
PB  - Wiley-VCH Verlag GmbH
SN  - 10400397
KW  - Linear scan voltametry
KW  - Elimination voltammetry
KW  - Irreversible current
KW  - Adsorptive Stripping Voltammetry
KW  - DNA
N2  - Adsorptive stripping voltammetry has been extended by elimination voltammetry with the linear scan rate (EVLS). EVLS offers the elimination of selected currents from the linear scan voltammetry results. It can be achieved by an elimination function formed by a linear combination of total currents measured at different scan rates. The problem of substance adsorption influence on the elimination voltammetry results has been studied. Theoretical curves of an irreversible current with adsorbed substance being electroactive were calculated for three elimination functions. As a practical application, the adsorptive stripping voltammetry of thermally denatured DNA on a hanging mercury electrode has been developed. While the LSV signal of single-stranded DNA at low concentrations gives a slight hint of the cathodic peak (due to the reduction of adenine and cytosine residues), the elimination provides quite a clear signal in the form of the peak-counterpeak. It is possible to determinate DNA at concentrations below of micrograms per milliliter.
ER  -

TRNKOVÁ, Libuše, René KIZEK a Oldřich DRAČKA. Application of Elimination Voltammetry to Adsorptive Stripping of DNA. \textit{Electroanalysis}. Weinheim: Wiley-VCH Verlag GmbH, 2000, roč.~2000, č.~12, s.~905-911. ISSN~1040-0397.

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