Characterization of oxacillin-resistant coagulase-negative
staphylococcal strains isolated in the Czech Republic

a 2000

Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic

PANTŮČEK, Roman, Václav HÁJEK, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC et. al.

Základní údaje

Originální název

Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic

Autoři

PANTŮČEK, Roman (203 Česká republika), Václav HÁJEK, Ivo SEDLÁČEK (203 Česká republika), Petr PETRÁŠ, Pavel ŠVEC (203 Česká republika), Vladislava RŮŽIČKOVÁ (203 Česká republika), Stanislav ROSYPAL (203 Česká republika), Jana KAILEROVÁ (203 Česká republika), Jitka FAITOVÁ (203 Česká republika), Eva ZAHRADNÍČKOVÁ (203 Česká republika), Roman RYBÁŘ (203 Česká republika) a Jiří DOŠKAŘ (203 Česká republika)

Vydání

9th International Symposium on Staphylococci and Staphylococcal Infections, 2000

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Dánsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00002296

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

coagulase negative staphylococci

Změněno: 31. 3. 2010 11:59, prof. RNDr. Ivo Sedláček, CSc.

Anotace

V originále

The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the differences in the restriction patterns of all the strains exhibiting similarity 60-95%. On the other hand the ribotyping with the probe 16S-23S rDNA from E. coli led to the estimation of 4 distinct ribotypes only. The PFGE-heterogeneity of a lower degree (similarity 70-100%) was found in the strains S. haemolyticus and S. hominis, which correlates well with the estimated ribotypes. Almost each of the S. haemolyticus strains exhibited a characteristic plasmid profile corresponding to the differences observed in the antibiotics resistance patterns. Seven or 8 different plasmid profiles were found to occur in S. epidermidis and S. hominis strains, respectively. It can be concluded that the strains of all three species under study are characteristic of a considerable genomic variability. For their differentation the PFGE proved to have the highest discriminatory power. Plasmid analysis appears to be suitable as typing method for S. haemolyticus.

Návaznosti

GA301/99/D075, projekt VaV

Název: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

Investor: Grantová agentura ČR, Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků

MSM 143100008, záměr

Název: Genomy a jejich funkce

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Genomy a jejich funkce

Citovat

PANTŮČEK, Roman, Václav HÁJEK, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Jana KAILEROVÁ, Jitka FAITOVÁ, Eva ZAHRADNÍČKOVÁ, Roman RYBÁŘ a Jiří DOŠKAŘ. Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic. In 9th International Symposium on Staphylococci and Staphylococcal Infections. 2000.

@proceedings{327191,
   author = {Pantůček, Roman and Hájek, Václav and Sedláček, Ivo and Petráš, Petr and Švec, Pavel and Růžičková, Vladislava and Rosypal, Stanislav and Kailerová, Jana and Faitová, Jitka and Zahradníčková, Eva and Rybář, Roman and Doškař, Jiří},
   booktitle = {9th International Symposium on Staphylococci and Staphylococcal Infections},
   keywords = {coagulase negative staphylococci},
   language = {eng},
   title = {Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic},
   year = {2000}
}

TY  - CONF
ID  - 327191
AU  - Pantůček, Roman - Hájek, Václav - Sedláček, Ivo - Petráš, Petr - Švec, Pavel - Růžičková, Vladislava - Rosypal, Stanislav - Kailerová, Jana - Faitová, Jitka - Zahradníčková, Eva - Rybář, Roman - Doškař, Jiří
PY  - 2000
TI  - Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic
KW  - coagulase negative staphylococci
N2  - The aim of this study was to estimate the genetic variability of 21 Staphylococcus epidermidis, 20 Staphylococcus haemolyticus and 30 Staphylococcus hominis strains resistant to oxacillin and multiresistant to antibiotics isolated from various clinical materials in Czech Republic during 1996-1999. Identification of these strains was carried out by estimating their biochemical features by use of STAPHYTest 16 and the ID32 Staph systems. In all the strains the resistance to 15 antibiotics was tested. The phage-typing was performed in using the Lyon and London phage sets at 100xRTD as well as the polyvalent phages and their host-range mutants. Oxacillin resistance was confirmed by the gene mecA PCR-amplification. For purpose of estimating genetic relationships in all the strains under study the SmaI-macrorestriction analysis, ribotyping, plasmid content and PCR-amplification of spacer region in 16S-23S rDNA were carried out. The results of SmaI-macrorestriction analysis in S. epidermidis indicated the differences in the restriction patterns of all the strains exhibiting similarity 60-95%. On the other hand the ribotyping with the probe 16S-23S rDNA from E. coli led to the estimation of 4 distinct ribotypes only. The PFGE-heterogeneity of a lower degree (similarity 70-100%) was found in the strains S. haemolyticus and S. hominis, which correlates well with the estimated ribotypes. Almost each of the S. haemolyticus strains exhibited a characteristic plasmid profile corresponding to the differences observed in the antibiotics resistance patterns. Seven or 8 different plasmid profiles were found to occur in S. epidermidis and S. hominis strains, respectively. It can be concluded that the strains of all three species under study are characteristic of a considerable genomic variability. For their differentation the PFGE proved to have the highest discriminatory power. Plasmid analysis appears to be suitable as typing method for S. haemolyticus.
ER  -

PANTŮČEK, Roman, Václav HÁJEK, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC, Vladislava RŮŽIČKOVÁ, Stanislav ROSYPAL, Jana KAILEROVÁ, Jitka FAITOVÁ, Eva ZAHRADNÍČKOVÁ, Roman RYBÁŘ a Jiří DOŠKAŘ. Characterization of oxacillin-resistant coagulase-negative staphylococcal strains isolated in the Czech Republic. In \textit{9th International Symposium on Staphylococci and Staphylococcal Infections}. 2000.

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