J 2000

Sequence specificity, conformation, and recognition by HMG1 protein of major DNA interstrand cross-links of antitumor dinuclear platinum complexes

KAŠPÁRKOVÁ, Jana, Nicholas FARRELL and Viktor BRABEC

Basic information

Original name

Sequence specificity, conformation, and recognition by HMG1 protein of major DNA interstrand cross-links of antitumor dinuclear platinum complexes

Authors

KAŠPÁRKOVÁ, Jana, Nicholas FARRELL and Viktor BRABEC

Edition

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2000, 0021-9258

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 7.368

RIV identification code

RIV/00216224:14310/00:00003308

Organization unit

Faculty of Science
Změněno: 27/2/2001 09:57, prof. RNDr. Viktor Brabec, DrSc.

Abstract

V originále

Interactions of high mobility group (HMG)-domain proteins with DNA modified by cisplatin plays a role in mechanisms underlying its antitumor activity. A structural motif recognized by HMG-domain proteins on cisplatin-modified DNA is a stable, directional bend of the helix axis. In the present work, bending induced in DNA by major adducts of a novel class of antitumor compounds, represented by the formula [{trans-[PtCl(NH3)2}H2N(CH2)2-6NH2]Cl2, was investigated. Gel retardation assay revealed very weak recognition of DNA adducts of dinuclear complexes by HMG1 protein. Hence, the mediation of antitumor properties of dinuclear platinum complexes by HMG-domain proteins is unlikely so that polynuclear platinum compounds may represent a novel class of platinum anticancer drugs acting by a different mechanism than cisplatin and its analogues.

Links

GA305/99/0695, research and development project
Name: Ovlivnění konformace DNA protinádorově účinnými komplexy kovů. Vztah k vývoji nových cytostatik.