Capillary Electrochromatography in Polyacrylamide Based
Monolithic Capillary Columns

D 2000

Capillary Electrochromatography in Polyacrylamide Based Monolithic Capillary Columns

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA, Pavel MUSIL et. al.

Základní údaje

Originální název

Capillary Electrochromatography in Polyacrylamide Based Monolithic Capillary Columns

Autoři

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL

Vydání

Bratislava, Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000, s. 85-85, 2000

Nakladatel

Slovenská chemická společnost

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00003323

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Capillary electrochromatography; Monolothic columns; Lignans; Schizandra

Štítky

Capillary electrochromatography, lignans, Monolothic columns, Schizandra

Změněno: 27. 2. 2001 10:54, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Capillary electrochromatography (CEC) is a hybrid separation technique that combines the benefits of both capillary zone electrophoresis and high performance liquid chromatography. The high efficiency and resolution of CEC offers an alternative to pressure-driven chromatography systems. Polymer based monolithic capillary columns quickly become a well-established stationary phases in CEC as they eliminated the need of frits and packing procedures. The large variety of available chemistries make these columns an attractive alternative to columns packed with particulate materials. The macroporous polyacrylamide columns for CEC were prepared by in situ copolymerisation of acrylamide, bisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of polyethylene glycol as a porogenic agent. The columns were successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 20 min. The migration time and peak area reproducibilities were better than 4 %. The results were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in good agreement with those determined by HPLC.

Návaznosti

GA303/00/D062, projekt VaV

Název: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Grantová agentura ČR, Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Citovat

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL. Capillary Electrochromatography in Polyacrylamide Based Monolithic Capillary Columns. In Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000. Bratislava: Slovenská chemická společnost, 2000, s. 85.

@inproceedings{331271,
   author = {Kvasničková, Leona and Glatz, Zdeněk and Kahle, Vladislav and Slanina, Jiří and Musil, Pavel},
   address = {Bratislava},
   booktitle = {Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000},
   keywords = {Capillary electrochromatography; Monolothic columns; Lignans; Schizandra},
   language = {eng},
   location = {Bratislava},
   pages = {85-85},
   publisher = {Slovenská chemická společnost},
   title = {Capillary Electrochromatography in Polyacrylamide Based Monolithic Capillary Columns},
   year = {2000}
}

TY  - JOUR
ID  - 331271
AU  - Kvasničková, Leona - Glatz, Zdeněk - Kahle, Vladislav - Slanina, Jiří - Musil, Pavel
PY  - 2000
TI  - Capillary Electrochromatography in Polyacrylamide Based Monolithic Capillary Columns
PB  - Slovenská chemická společnost
CY  - Bratislava
KW  - Capillary electrochromatography
KW  - Monolothic columns
KW  - Lignans
KW  - Schizandra
N2  - Capillary electrochromatography (CEC) is a hybrid separation technique that combines the benefits of both capillary zone electrophoresis and high performance liquid chromatography. The high efficiency and resolution of CEC offers an alternative to pressure-driven chromatography systems. Polymer based monolithic capillary columns quickly become a well-established stationary phases in CEC as they eliminated the need of frits and packing procedures. The large variety of available chemistries make these columns an attractive alternative to columns packed with particulate materials. The macroporous polyacrylamide columns for CEC were prepared by in situ copolymerisation of acrylamide, bisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of polyethylene glycol as a porogenic agent. The columns were successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 20 min. The migration time and peak area reproducibilities were better than 4 %. The results were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in good agreement with those determined by HPLC.
ER  -

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL. Capillary Electrochromatography in Polyacrylamide Based Monolithic Capillary Columns. In \textit{Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000}. Bratislava: Slovenská chemická společnost, 2000, s.~85.

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