Determination of Phenolic Compounds in Artychoke Exctract by
Means of Capillary Zone Electrophoresis

D 2000

Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL

Základní údaje

Originální název

Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis

Autoři

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL

Vydání

Bratislava, Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000, s. 85-85, 2000

Nakladatel

Slovenská chemická společnost

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00003324

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Capillary electrophoresis; Artychoke; Phenols

Štítky

Artychoke, Capillary electrophoresis, Phenols

Změněno: 27. 2. 2001 11:01, prof. RNDr. Zdeněk Glatz, CSc.

Anotace

V originále

Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives.

Návaznosti

GA303/00/D062, projekt VaV

Název: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Grantová agentura ČR, Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Citovat

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL. Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis. In Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000. Bratislava: Slovenská chemická společnost, 2000, s. 85.

@inproceedings{331351,
   author = {Ševčíková, Petra and Glatz, Zdeněk and Slanina, Jiří and Musil, Pavel},
   address = {Bratislava},
   booktitle = {Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000},
   keywords = {Capillary electrophoresis; Artychoke; Phenols},
   language = {eng},
   location = {Bratislava},
   pages = {85-85},
   publisher = {Slovenská chemická společnost},
   title = {Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis},
   year = {2000}
}

TY  - JOUR
ID  - 331351
AU  - Ševčíková, Petra - Glatz, Zdeněk - Slanina, Jiří - Musil, Pavel
PY  - 2000
TI  - Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis
PB  - Slovenská chemická společnost
CY  - Bratislava
KW  - Capillary electrophoresis
KW  - Artychoke
KW  - Phenols
N2  - Artichoke (Cynara) is well known as a vegetable, but it has also application in pharmacology. The choleretic and hypocholesterolemic actions of the artichoke extract are already known. The present research is being carried out into the antioxidative and hepatoprotective effects of such extracts. These activities have been attributed to the phenolic compounds present in the extract and to its caffeoylquinic derivatives. Micellar electrokinetic capillary chromatography (MECC) has been developed as a promising method for the analysis of the Artichoke extract. Separation conditions have been optimised with respect to different parameters including pH and ion strength of the background electrolyte, SDS concentration and temperature. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.5 was found to be the most suitable electrolyte for this separation. An applied voltage of 28 kV (positive polarity) and a temperature of 25 oC gave the best separation of the caffeoylquinic acid derivatives.
ER  -

ŠEVČÍKOVÁ, Petra, Zdeněk GLATZ, Jiří SLANINA a Pavel MUSIL. Determination of Phenolic Compounds in Artychoke Exctract by Means of Capillary Zone Electrophoresis. In \textit{Book of Abstract, International Symposium on Capillary Electrophoresis, ITP 2000}. Bratislava: Slovenská chemická společnost, 2000, s.~85.

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