Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy.

KLUSÁKOVÁ, Ilona, Petr DUBOVÝ and Ivana SVÍŽENSKÁ. Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy. In Sborník lékařský. Praha: Karlova Univerzita, 2000, p. 14. Vol. 101, No. 4.

Basic information

• Original name: Immunohistochemical study of proliferation and apoptosis in the dorsal root ganglia after supraspinal and infraspinal axotomy.
• Authors: KLUSÁKOVÁ, Ilona, Petr DUBOVÝ and Ivana SVÍŽENSKÁ.
• Edition: Praha, Sborník lékařský, p. 14-14, Vol. 101, No. 4, 2000.
• Publisher: Karlova Univerzita

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/00:00002597
• Organization unit: Faculty of Medicine
• Keywords in English: spinal ganglia; TUNEL; apoptosis; axotomy
• Tags: apoptosis, axotomy, spinal ganglia, TUNEL

Abstract

Disconnection of the peripheral axonal branch from the target tissue results in changes of GFAP expression in the satellite cells of the dorsal root ganglia (DRG) and their proliferation The loss of DRG neurons was also described after the peripheral axotomy. No results are available about the reaction of DRG neurons and their satellite cells to the central axotomy. We have investigated proliferation and apoptosis in the adult rat DRG after central and peripheral axotomy. Female rats were operated for the central axotomy and peripheral axotomy. The animals were perfused through ascending aorta with phosphate-buffered saline followed Zamboni's fixative solution after 2, 4, and 6 weeks. Cryostat sections through affected and intact DRG were incubated for immunohistochemical identification of BrdU as well as apoptosis (TUNEL). Both peripheral and central axotomy stimulated proliferation of many satellite cells while apoptosis was observed in lower amount of the cells.

Links

• GA309/00/0407, research and development project: Name: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů

Investor: Czech Science Foundation, Cellular and extracellular environment for the axon regenaration following injury of the spinal roots and peripheral nerves