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@article{346311,
author = {Kozubek, Stanislav and Lukášová, Emilie and Amrichová, Jana and Kozubek, Michal and Lišková, Andrea and Šlotová, Jana},
article_location = {San Diego},
article_number = {1},
keywords = {chromatin structure; cell fixation; confocal laser scanning microscopy; image analysis; interphase nucleus},
language = {eng},
issn = {0003-2697},
journal = {Analytical Biochemistry},
title = {Influence of cell fixation on chromatin topography},
url = {http://www.fi.muni.cz/lom/abstracts/lom006.shtml},
volume = {282},
year = {2000}
}
TY - JOUR
ID - 346311
AU - Kozubek, Stanislav - Lukášová, Emilie - Amrichová, Jana - Kozubek, Michal - Lišková, Andrea - Šlotová, Jana
PY - 2000
TI - Influence of cell fixation on chromatin topography
JF - Analytical Biochemistry
VL - 282
IS - 1
SP - 29
EP - 29
PB - Academic Press
SN - 00032697
KW - chromatin structure
KW - cell fixation
KW - confocal laser scanning microscopy
KW - image analysis
KW - interphase nucleus
UR - http://www.fi.muni.cz/lom/abstracts/lom006.shtml
N2 - Using in situ hybridization techniques, a fixation step must precede denaturation to prevent disintegration of the chromosomes. The analysis of nuclei fixed by paraformaldehyde, preserving the native structure (three-dimensional or 3D fixation and analysis) has become possible with the development of confocal microscopy; however, the analysis of those fixed by methanol and acetic acid, dehydrating the nuclei (two-dimensional or 2D fixation and analysis), remains a very valuable tool for practical use in diagnostics and also in many cases for research. We compared both types of fixation and analyses using different cell lines and different DNA probes. Fixation of cells by methanol and acetic acid leads to the enlargement of contact of nuclei with the slide surface, resulting in a substantial increase of nuclear diameter, flattening of the nucleus, and consequently to a distortion of gene topology.
ER -
KOZUBEK, Stanislav, Emilie LUKÁŠOVÁ, Jana AMRICHOVÁ, Michal KOZUBEK, Andrea LIŠKOVÁ and Jana ŠLOTOVÁ. Influence of cell fixation on chromatin topography. \textit{Analytical Biochemistry}. San Diego: Academic Press, 2000, vol.~282, No~1, p.~29-38. ISSN~0003-2697.
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