Další formáty:
BibTeX
LaTeX
RIS
@article{346311, author = {Kozubek, Stanislav and Lukášová, Emilie and Amrichová, Jana and Kozubek, Michal and Lišková, Andrea and Šlotová, Jana}, article_location = {San Diego}, article_number = {1}, keywords = {chromatin structure; cell fixation; confocal laser scanning microscopy; image analysis; interphase nucleus}, language = {eng}, issn = {0003-2697}, journal = {Analytical Biochemistry}, title = {Influence of cell fixation on chromatin topography}, url = {http://www.fi.muni.cz/lom/abstracts/lom006.shtml}, volume = {282}, year = {2000} }
TY - JOUR ID - 346311 AU - Kozubek, Stanislav - Lukášová, Emilie - Amrichová, Jana - Kozubek, Michal - Lišková, Andrea - Šlotová, Jana PY - 2000 TI - Influence of cell fixation on chromatin topography JF - Analytical Biochemistry VL - 282 IS - 1 SP - 29 EP - 29 PB - Academic Press SN - 00032697 KW - chromatin structure KW - cell fixation KW - confocal laser scanning microscopy KW - image analysis KW - interphase nucleus UR - http://www.fi.muni.cz/lom/abstracts/lom006.shtml N2 - Using in situ hybridization techniques, a fixation step must precede denaturation to prevent disintegration of the chromosomes. The analysis of nuclei fixed by paraformaldehyde, preserving the native structure (three-dimensional or 3D fixation and analysis) has become possible with the development of confocal microscopy; however, the analysis of those fixed by methanol and acetic acid, dehydrating the nuclei (two-dimensional or 2D fixation and analysis), remains a very valuable tool for practical use in diagnostics and also in many cases for research. We compared both types of fixation and analyses using different cell lines and different DNA probes. Fixation of cells by methanol and acetic acid leads to the enlargement of contact of nuclei with the slide surface, resulting in a substantial increase of nuclear diameter, flattening of the nucleus, and consequently to a distortion of gene topology. ER -
KOZUBEK, Stanislav, Emilie LUKÁŠOVÁ, Jana AMRICHOVÁ, Michal KOZUBEK, Andrea LIŠKOVÁ a Jana ŠLOTOVÁ. Influence of cell fixation on chromatin topography. \textit{Analytical Biochemistry}. San Diego: Academic Press, 2000, roč.~282, č.~1, s.~29-38. ISSN~0003-2697.
|