An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy

KOZUBEK, Michal and Petr MATULA. An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy. Journal of Microscopy. Oxford: Blackwell Science, 2000, vol. 200, No 3, p. 206-217. ISSN 0022-2720.

Basic information

• Original name: An efficient algorithm for measurement and correction of chromatic aberrations in fluorescence microscopy
• Authors: KOZUBEK, Michal (203 Czech Republic, guarantor, belonging to the institution) and Petr MATULA (203 Czech Republic, belonging to the institution).
• Edition: Journal of Microscopy, Oxford, Blackwell Science, 2000, 0022-2720.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 20200 2.2 Electrical engineering, Electronic engineering, Information engineering
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 1.173
• RIV identification code: RIV/00216224:14330/00:00002780
• Organization unit: Faculty of Informatics
• UT WoS: 000165550500004
• Keywords in English: chromatic aberration; fluorescence microscopy; fluorescent bead; image analysis
• Tags: cbia-web, chromatic aberration, fluorescence microscopy, fluorescent bead, Image analysis
• Tags: International impact, Reviewed

Abstract

Even the best optical microscopes available on the market exhibit chromatic aberrations to some extent. In some types of study, chromatic aberrations of current optics cannot be neglected and a software correction is highly desirable. This paper describes a novel method of chromatic aberration measurement and software correction using sub-resolution bead imaging and computer image analysis. The method is quick, precise and enables the determination of both longitudinal and lateral chromatic aberrations. Correction function can be computed in about half an hour, including image acquisition. Using this approach, chromatic aberrations can be reduced to 10-20 nm laterally and 10-60 nm axially depending on the type of optical set-up. The method is especially suitable for fluorescence microscopy, where a limited number of wavelengths are observed.

Links

• GA202/99/P008, research and development project: Name: Využití analýzy obrazu při studiu struktury interfázního jádra

Investor: Czech Science Foundation, Image analysis in the study of interphase nucleus structure

• IBS5004010, research and development project: Name: Vývoj nových diagnostických technik pro onkologii

Investor: Academy of Sciences of the Czech Republic, Development of new diagnostic teniques for oncology

• MSM 143300002, plan (intention): Name: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy

• VS97031, research and development project: Name: Využití analýzy obrazu při studiu mechanismů vzniku, v diagnostice a pro prevenci závažných onemocnění člověka

Investor: Ministry of Education, Youth and Sports of the CR, Image analysis in the study of mechanisms of induction, in diagnosis and for prevention of deleterious human diseases