Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond
delayed and prompt chlorophyll fluorescence of Dianthus
caryophyllus L. axillary buds cultured in vitro

J 2001

Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro

GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA and Iordanka IVANOVA

Basic information

Original name

Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro

Authors

GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA and Iordanka IVANOVA

Edition

Scientia Horticulturae, Amsterdam, Elsevier, 2001, 0304-4238

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 0.510

RIV identification code

RIV/00216224:14310/01:00004047

Organization unit

Faculty of Science

UT WoS

000169276000004

Keywords in English

cytokinins; Dianthus caryophyllus; 4-PU-30; thidiazuron; photosystem II; chlorophyll fluorescence; delayed fluorescence

Abstract

V originále

The effects of benzyladenine (BA) and two phenylurea cytokinins (N-phenyl-N'-(2-chloro-4-pyridyl)urea (4-PU-30) and thidiazuron (TDZ)) on in vitro cultures of carnation were studied using induction kinetics and thermograms of prompt and delayed chlorophyll fluorescence. In plantlets obtained from explants cultivated on a medium with 0.4 microM BA or 0.04-0.004 microM phenylurea cytokinins, the photosynthetic apparatus showed high PS II photochemical activity comparable to control without cytokinins and 5.8-97.5% higher estimated photoinduced transmembrane proton gradient (low concentrations of 4-PU-30 are especially effective). At a concentration of 0.4 microM, when compared to BA treatment, phenylurea cytokinins induced 24-35% suppression of photochemical activity, 44-46% suppression of estimated transmembrane proton gradient and 12-17% suppression of estimated electron transport rate. It is likely that this concentration of phenylurea cytokinins exceeded the optimal dose. The analysis of the same luminescent characteristics in the presence of different 4-PU-30 and TDZ concentrations showed that these compounds induced dose-dependent changes in the functional activity as well as alteration in the level of energization of the thylakoid membrane. When fluorescence thermograms were evaluated, phenylureas appeared to change the high temperature stability of the photosynthetic apparatus.

Links

MSM 143100008, plan (intention)

Name: Genomy a jejich funkce

Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions

Citovat

GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA and Iordanka IVANOVA. Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro. Scientia Horticulturae. Amsterdam: Elsevier, 2001, vol. 89, No 1, p. 41-55. ISSN 0304-4238.

@article{346403,
   author = {Goltsev, Vasiliij and Genkov, Todor Nikolaev and Lexa, Matej and Ivanova, Iordanka},
   article_location = {Amsterdam},
   article_number = {1},
   keywords = {cytokinins; Dianthus caryophyllus; 4-PU-30; thidiazuron; photosystem II; chlorophyll fluorescence; delayed fluorescence},
   language = {eng},
   issn = {0304-4238},
   journal = {Scientia Horticulturae},
   title = {Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro},
   volume = {89},
   year = {2001}
}

TY  - JOUR
ID  - 346403
AU  - Goltsev, Vasiliij - Genkov, Todor Nikolaev - Lexa, Matej - Ivanova, Iordanka
PY  - 2001
TI  - Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro
JF  - Scientia Horticulturae
VL  - 89
IS  - 1
SP  - 41
EP  - 41
PB  - Elsevier
SN  - 03044238
KW  - cytokinins
KW  - Dianthus caryophyllus
KW  - 4-PU-30
KW  - thidiazuron
KW  - photosystem II
KW  - chlorophyll fluorescence
KW  - delayed fluorescence
N2  - The effects of benzyladenine (BA) and two phenylurea cytokinins (N-phenyl-N'-(2-chloro-4-pyridyl)urea (4-PU-30) and thidiazuron (TDZ)) on in vitro cultures of carnation were studied using induction kinetics and thermograms of prompt and delayed chlorophyll fluorescence. In plantlets obtained from explants cultivated on a medium with 0.4 microM BA or 0.04-0.004 microM phenylurea cytokinins, the photosynthetic apparatus showed high PS II photochemical activity comparable to control without cytokinins and 5.8-97.5% higher estimated photoinduced transmembrane proton gradient (low concentrations of 4-PU-30 are especially effective). At a concentration of 0.4 microM, when compared to BA treatment, phenylurea cytokinins induced 24-35% suppression of photochemical activity, 44-46% suppression of estimated transmembrane proton gradient and 12-17% suppression of estimated electron transport rate. It is likely that this concentration of phenylurea cytokinins exceeded the optimal dose. The analysis of the same luminescent characteristics in the presence of different 4-PU-30 and TDZ concentrations showed that these compounds induced dose-dependent changes in the functional activity as well as alteration in the level of energization of the thylakoid membrane. When fluorescence thermograms were evaluated, phenylureas appeared to change the high temperature stability of the photosynthetic apparatus.
ER  -

GOLTSEV, Vasiliij, Todor Nikolaev GENKOV, Matej LEXA and Iordanka IVANOVA. Effect of benzyladenine, 4-PU-30 and thidiazuron on millisecond delayed and prompt chlorophyll fluorescence of Dianthus caryophyllus L. axillary buds cultured in vitro. \textit{Scientia Horticulturae}. Amsterdam: Elsevier, 2001, vol.~89, No~1, p.~41-55. ISSN~0304-4238.

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