| MARVANOVÁ, Soňa, Yuji NAGATA, Michaela WIMMEROVÁ, Jana SÝKOROVÁ, Kamila HYNKOVÁ a Jiří DAMBORSKÝ. Biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants. Journal of Microbiological Methods. 2001, roč. 44, č. 1, s. 149-157. ISSN 0167-7012. |
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@article{352155,
author = {Marvanová, Soňa and Nagata, Yuji and Wimmerová, Michaela and Sýkorová, Jana and Hynková, Kamila and Damborský, Jiří},
article_number = {1},
language = {eng},
issn = {0167-7012},
journal = {Journal of Microbiological Methods},
title = {Biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants},
url = {http://www.ncbr.chemi.muni.cz/~jiri/ABSTRACTS/jmm01.html},
volume = {44},
year = {2001}
}
TY - JOUR
ID - 352155
AU - Marvanová, Soňa - Nagata, Yuji - Wimmerová, Michaela - Sýkorová, Jana - Hynková, Kamila - Damborský, Jiří
PY - 2001
TI - Biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants
JF - Journal of Microbiological Methods
VL - 44
IS - 1
SP - 149
EP - 149
SN - 01677012
UR - http://www.ncbr.chemi.muni.cz/~jiri/ABSTRACTS/jmm01.html
N2 - The pH indicator dye-based colorimetric method and multivariate experimental design were used for the systematic biochemical characterization of the broad-specificity enzymes haloalkane dehalogenases. Halogenated compounds for characterization of the enzymes were selected using Principal Component Analysis. The substrates were characterised by 24 physico-chemical and structural descriptors. Thirty four substrates were selected for testing out of 194 halogenated compounds. Relative activities determined using the optimised colorimetric microplate assay were validated against the catalytic constants determined by gas chromatography. The applicability of the assay was tested with F151L, F154L and F169L mutants of the haloalkane dehalogenase from Sphingomonas paucimobilis UT26.
ER -
MARVANOVÁ, Soňa, Yuji NAGATA, Michaela WIMMEROVÁ, Jana SÝKOROVÁ, Kamila HYNKOVÁ a Jiří DAMBORSKÝ. Biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants. \textit{Journal of Microbiological Methods}. 2001, roč.~44, č.~1, s.~149-157. ISSN~0167-7012.
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