1999
Apoptosis in pig leucocytes during cardiopulmonary bypass
MATALOVÁ, Eva, Alena ŠPANOVÁ, Bohuslav RITTICH, Robert WAGNER, František KOVÁŘŮ et. al.Základní údaje
Originální název
Apoptosis in pig leucocytes during cardiopulmonary bypass
Autoři
MATALOVÁ, Eva, Alena ŠPANOVÁ, Bohuslav RITTICH, Robert WAGNER a František KOVÁŘŮ
Vydání
New Mexico, USA, Inflammatory Paradigms and the Vasculature, s. 34-34, Abstract Book, 1999
Nakladatel
Keystone Symposia
Další údaje
Jazyk
angličtina
Typ výsledku
Stať ve sborníku
Obor
Genetika a molekulární biologie
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/99:00004085
Organizační jednotka
Přírodovědecká fakulta
Změněno: 29. 3. 2001 11:03, doc. RNDr. Alena Španová, CSc.
Anotace
V originále
The aim of this study was to observe programmed cell death (PCD) activation in pig leucocytes durind cardiopulmonary bypass (CPB). Blood samples were taken prior to, during, and after CPB. Apoptosis was detected using TUNEL assay and by typical apoptotic morphology changes (Giemsa staining). Apoptotic DNA fragmentation was proved in high percentage of cells in the operating beginning. A decrease in the number of apoptotic cells was detected in the surviving pigs after the aorta clip release. On the other hand, the number of apoptotic leucocytes did not significantly change in pigs died owing to postperfusion syndrom. The same alterations in percentage of apoptotic cells were proved in bone marrow after chest opening, after surgery and in dead animals. These results suggested that PCD can play a role in the postperfusion syndrome after CPB.
Návaznosti
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