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@inproceedings{355857,
author = {Vetterl, Vladimír and Jelen, František and Hasoň, Stanislav and Běluša, Petr},
address = {Praha},
booktitle = {Book of Abstracts, J.Heyrovský Memorial Symposium, Praha},
keywords = {DNA adsorption; electrode double layer admittance; stripping analysis},
language = {eng},
location = {Praha},
pages = {44-44},
publisher = {Ústav fyzikální chemie J.Heyrovského, AV ČR},
title = {ADSORPTIVE STRIPPING ANALYSIS OF DAMAGE DNA WITH ADMITTANCE MEASUREMENTS},
year = {2000}
}
TY - JOUR
ID - 355857
AU - Vetterl, Vladimír - Jelen, František - Hasoň, Stanislav - Běluša, Petr
PY - 2000
TI - ADSORPTIVE STRIPPING ANALYSIS OF DAMAGE DNA WITH ADMITTANCE MEASUREMENTS
PB - Ústav fyzikální chemie J.Heyrovského, AV ČR
CY - Praha
KW - DNA adsorption
KW - electrode double layer admittance
KW - stripping analysis
N2 - Native, denatured and damaged calf thymus DNA was studied by admittance measurements using hanging mercury drop electrode. We used adsorptive stripping technique with application of forward (negative-going) and reverse (positive-going) potential scan. The effect of alternating current voltage, frequency, adsorption potential, voltage amplitude and adsorption time was studied. Native double-stranded (ds) DNA produced peak 3 and inflexion 2 on the forward but not on the reverse curves. Irradiation of ds DNA with relatively small doses of g-radiation (up to 80 Gy) resulted in an appearance of peak 3 and 2 on reverse voltammetric curves. Similar peaks were observed when ds DNA was sonicated. These results suggest that in damaged regions of ds DNA bases are accessible for the interaction with the electrode producing peak 3 without passing through the potential region U around -1.2 V, where ds DNA is supposed to be slowly opened.
ER -
VETTERL, Vladimír, František JELEN, Stanislav HASOŇ a Petr BĚLUŠA. ADSORPTIVE STRIPPING ANALYSIS OF DAMAGE DNA WITH ADMITTANCE MEASUREMENTS. In \textit{Book of Abstracts, J.Heyrovský Memorial Symposium, Praha}. Praha: Ústav fyzikální chemie J.Heyrovského, AV ČR, 2000, s.~44.
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