Electrochemical impedance spectroscopy of native and denatured
DNA.

D 2000

Electrochemical impedance spectroscopy of native and denatured DNA.

VETTERL, Vladimír, František JELEN, Viktor DRAŽAN, Luděk STRAŠÁKA, Stanislav HASOŇ et. al.

Basic information

Original name

Electrochemical impedance spectroscopy of native and denatured DNA.

Authors

VETTERL, Vladimír (203 Czech Republic, guarantor), František JELEN, Viktor DRAŽAN, Luděk STRAŠÁKA and Stanislav HASOŇ

Edition

Brno, DNA Structure and Interaction - Gregor Mendel Symposium, Book of Abstracts, p. 9-9, 2000

Publisher

Biofyzikální ústav AV ČR

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14330/00:00004093

Organization unit

Faculty of Informatics

Keywords in English

DNA adsorption; electrode double layer; impedance spectroscopy

Abstract

V originále

From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. The complex plane impedance plots for DNA exhibited arc shapes, from which the apparent resistance R2 of the layer and the solution resistance R1 was determined. The resistance R2 represents the dielectric losses of the capacitance of the electrode double layer, i.e. the energy lost as a heat which arises due to a friction of charged DNA segments forced to move in a viscous solvent by a.c. electric field. It was found that with DNA solutions the dielectric losses are higher (i.e. the resistance R2 is lower) at the potentials where the desorption takes place than at the potentials of maximum adsorption. The desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of denatured DNA compared to the dsDNA resulting in the higher dielectric losses.

Links

GA203/00/P081, research and development project

Name: Adsorpce bází nukleových kyselin a jejich derivátů na elektrodách

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

IAA4004002, research and development project

Name: Struktura a interakce nukleových kyselin a polypeptidů na površích kovů

Investor: Academy of Sciences of the Czech Republic, Structure and Interaction of Nucleic Acids on Metal Surfaces

Citovat

VETTERL, Vladimír, František JELEN, Viktor DRAŽAN, Luděk STRAŠÁKA and Stanislav HASOŇ. Electrochemical impedance spectroscopy of native and denatured DNA. In DNA Structure and Interaction - Gregor Mendel Symposium, Book of Abstracts. Brno: Biofyzikální ústav AV ČR, 2000, p. 9.

@inproceedings{355891,
   author = {Vetterl, Vladimír and Jelen, František and Dražan, Viktor and Strašáka, Luděk and Hasoň, Stanislav},
   address = {Brno},
   booktitle = {DNA Structure and Interaction - Gregor Mendel Symposium, Book of Abstracts},
   keywords = {DNA adsorption; electrode double layer; impedance spectroscopy},
   language = {eng},
   location = {Brno},
   pages = {9-9},
   publisher = {Biofyzikální ústav AV ČR},
   title = {Electrochemical impedance spectroscopy of native and denatured DNA.},
   year = {2000}
}

TY  - JOUR
ID  - 355891
AU  - Vetterl, Vladimír - Jelen, František - Dražan, Viktor - Strašáka, Luděk - Hasoň, Stanislav
PY  - 2000
TI  - Electrochemical impedance spectroscopy of native and denatured DNA.
PB  - Biofyzikální ústav AV ČR
CY  - Brno
KW  - DNA adsorption
KW  - electrode double layer
KW  - impedance spectroscopy
N2  - From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNA layer was determined. The complex plane impedance plots for DNA exhibited arc shapes, from which the apparent resistance R2 of the layer and the solution resistance R1 was determined. The resistance R2 represents the dielectric losses of the capacitance of the electrode double layer, i.e. the energy lost as a heat which arises due to a friction of charged DNA segments forced to move in a viscous solvent by a.c. electric field. It was found that with DNA solutions the dielectric losses are higher (i.e. the resistance R2 is lower) at the potentials where the desorption takes place than at the potentials of maximum adsorption. The desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of denatured DNA compared to the dsDNA resulting in the higher dielectric losses.
ER  -

VETTERL, Vladimír, František JELEN, Viktor DRAŽAN, Luděk STRAŠÁKA and Stanislav HASOŇ. Electrochemical impedance spectroscopy of native and denatured DNA. In \textit{DNA Structure and Interaction - Gregor Mendel Symposium, Book of Abstracts}. Brno: Biofyzikální ústav AV ČR, 2000, p.~9.

Detailed Information on Publication Record