Cytoskeleton and morphological aberrations of septum formation
and cytokinesis in cps6 mutant of Schizosaccharomyces pombe

D 2000

Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe

GABRIEL, Miroslav, Junpei ISHIGURO a Marie KOPECKÁ

Základní údaje

Originální název

Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe

Autoři

GABRIEL, Miroslav, Junpei ISHIGURO a Marie KOPECKÁ

Vydání

1. vyd. Praha, VII. Cytoskeletální klub, od s. 11-11, 1 s. VIII. Cytoskeletální klub, 2000

Nakladatel

Ústav molekulární genetiky Akademie věd

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14110/00:00003572

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

fission yeast; cytoskeleton; ultrastructure; cps mutant

Štítky

cps mutant, Cytoskeleton, fission yeast, ultrastructure

Změněno: 28. 2. 2001 10:41, doc. MUDr. Miroslav Gabriel, CSc.

Anotace

V originále

Cps6 mutant of the fission yeast is defective in cytokinesis, resulting in the formation of multiseptated cells.It is also more sensitive to digesting reagents than the wild type cells, suggesting that the mutant wall is altered in its composition or organization. The aim of this work was to examine wall and septum organization at the ultrastructural level and reinvestigate cytoskeleton in relation to aberrant phenotype.The results showed that the wall was less compact and more spongy with predominance of microfibrillar component.Lateral wall and septa were thicker. Septa remained unseparated.Cytoskeleton was well developed. Cps6 mutation may interfere with the termination of the synthesis of lateral wall and septum. Gene rad25+ coding for one of 14--3-3 homologs that complements cps6 mutation may be involved in pathway of cell wall integrity and proliferation.

Návaznosti

GA204/00/0394, projekt VaV

Název: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

Investor: Grantová agentura ČR, Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce

GA310/00/0391, projekt VaV

Název: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.

Investor: Grantová agentura ČR, Cytoskelet u lidských patogenních kvasinkových mikroorganismů

Citovat

GABRIEL, Miroslav, Junpei ISHIGURO a Marie KOPECKÁ. Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe. In VII. Cytoskeletální klub. 1. vyd. Praha: Ústav molekulární genetiky Akademie věd, 2000, s. 11-11. VIII. Cytoskeletální klub.

@inproceedings{356531,
   author = {Gabriel, Miroslav and Ishiguro, Junpei and Kopecká, Marie},
   address = {Praha},
   booktitle = {VII. Cytoskeletální klub},
   edition = {1.},
   keywords = {fission yeast; cytoskeleton; ultrastructure; cps mutant},
   language = {eng},
   location = {Praha},
   pages = {11-11},
   publisher = {Ústav molekulární genetiky Akademie věd},
   title = {Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe},
   year = {2000}
}

TY  - JOUR
ID  - 356531
AU  - Gabriel, Miroslav - Ishiguro, Junpei - Kopecká, Marie
PY  - 2000
TI  - Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe
VL  - VIII. Cytoskeletální klub
PB  - Ústav molekulární genetiky Akademie věd
CY  - Praha
KW  - fission yeast
KW  - cytoskeleton
KW  - ultrastructure
KW  - cps mutant
N2  - Cps6 mutant of the fission yeast is defective in cytokinesis, resulting in the formation of multiseptated cells.It is also more sensitive to digesting reagents than the wild type cells, suggesting that the mutant wall is altered in its composition or organization. The aim of this work was to examine wall and septum organization at the ultrastructural level and reinvestigate cytoskeleton in relation to aberrant phenotype.The results showed that the wall was less compact and more spongy with predominance of microfibrillar component.Lateral wall and septa were thicker. Septa remained unseparated.Cytoskeleton was well developed. Cps6 mutation may interfere with the termination of the synthesis of lateral wall and septum. Gene rad25+ coding for one of 14--3-3 homologs that complements cps6 mutation may be involved in pathway of cell wall integrity and proliferation.
ER  -

GABRIEL, Miroslav, Junpei ISHIGURO a Marie KOPECKÁ. Cytoskeleton and morphological aberrations of septum formation and cytokinesis in cps6 mutant of Schizosaccharomyces pombe. In \textit{VII. Cytoskeletální klub}. 1. vyd. Praha: Ústav molekulární genetiky Akademie věd, 2000, s.~11-11. VIII. Cytoskeletální klub.

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