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@article{357876, author = {Preisler, J. and Hu, P. and Rejtar, T. and Karger, B. L. and Hu, Ping and Rejtar, Tomáš}, article_location = {Washington, D.C., USA}, article_number = {20}, keywords = {MALDI; CE; interface; matrix-assisted laser desorption; vacuum; deposition; peptides; proteins; proteome; proteomic; digest}, language = {eng}, issn = {0003-2700}, journal = {Analytical Chemistry}, title = {Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface}, volume = {72}, year = {2000} }
TY - JOUR ID - 357876 AU - Preisler, J. - Hu, P. - Rejtar, T. - Karger, B. L. - Hu, Ping - Rejtar, Tomáš PY - 2000 TI - Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface JF - Analytical Chemistry VL - 72 IS - 20 SP - 4785 EP - 4785 PB - American Chemical Society SN - 00032700 KW - MALDI KW - CE KW - interface KW - matrix-assisted laser desorption KW - vacuum KW - deposition KW - peptides KW - proteins KW - proteome KW - proteomic KW - digest N2 - An improved vacuum deposition interface for coupling capillary electrophoresis with MALDI/TOF MS has been developed. Liquid samples consisting of analyte and matrix were deposited on a moving tape in the evacuated source chamber of TOF mass spectrometer, enabling a day of continuous analysis without interruption. The vacuum deposition procedure was compared with the dried-droplet method, and it was found that vacuum deposition generated significantly more reproducible signal intensity, eliminating the need for the "sweet spot" searching. In addition, ion suppression caused by hydrophobicity differences in the analytes was reduced. In order to minimize ion suppression further, separation prior to MALDI/MS analysis was employed. The performance of capillary electrophoresis (CE) - MALDI/TOF MS using the vacuum deposition interface was evaluated with a peptide mixture injected at low femtomole levels. All peptides were baseline resolved with separation efficiencies in the range of 250,000 - 400,000 plates/m (2 - 3 second band halfwidth), demonstrating the high separation efficiency of the CE-MALDI/MS coupling. ER -
PREISLER, J., P. HU, T. REJTAR a B. L. KARGER. Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2000, roč.~72, č.~20, s.~4785-4795. ISSN~0003-2700.
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