Capillary electrophoresis - Matrix-Assisted Laser
Desorption/Ionization Time-of-Flight Mass Spectrometry Using a
vacuum deposition Interface

J 2000

Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface

PREISLER, J., P. HU, T. REJTAR a B. L. KARGER

Základní údaje

Originální název

Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface

Název česky

Kapilární elektroforéza - laserová desorpce/ionizace za účasti matrice s půletovou hmotnostní spektrometrií a rozhraním pro vakuové nanášení

Autoři

PREISLER, J. (203 Česká republika, garant), P. HU (840 Spojené státy), T. REJTAR (203 Česká republika) a B. L. KARGER (840 Spojené státy)

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2000, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.587

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000089924800003

Klíčová slova anglicky

MALDI; CE; interface; matrix-assisted laser desorption; vacuum; deposition; peptides; proteins; proteome; proteomic; digest

Štítky

CE, deposition, digest, interface, MALDI, matrix-assisted laser desorption, peptides, proteins, proteome, proteomic, vacuum

Změněno: 28. 6. 2009 00:02, prof. Mgr. Jan Preisler, Ph.D.

Anotace

ORIG CZ

V originále

An improved vacuum deposition interface for coupling capillary electrophoresis with MALDI/TOF MS has been developed. Liquid samples consisting of analyte and matrix were deposited on a moving tape in the evacuated source chamber of TOF mass spectrometer, enabling a day of continuous analysis without interruption. The vacuum deposition procedure was compared with the dried-droplet method, and it was found that vacuum deposition generated significantly more reproducible signal intensity, eliminating the need for the "sweet spot" searching. In addition, ion suppression caused by hydrophobicity differences in the analytes was reduced. In order to minimize ion suppression further, separation prior to MALDI/MS analysis was employed. The performance of capillary electrophoresis (CE) - MALDI/TOF MS using the vacuum deposition interface was evaluated with a peptide mixture injected at low femtomole levels. All peptides were baseline resolved with separation efficiencies in the range of 250,000 - 400,000 plates/m (2 - 3 second band halfwidth), demonstrating the high separation efficiency of the CE-MALDI/MS coupling.

Česky

Kapilární elektroforéza - laserová desorpce/ionizace za účasti matrice s půletovou hmotnostní spektrometrií a rozhraním pro vakuové nanášení, viz anglický popis.

Citovat

PREISLER, J., P. HU, T. REJTAR a B. L. KARGER. Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2000, roč. 72, č. 20, s. 4785-4795. ISSN 0003-2700.

@article{357876,
   author = {Preisler, J. and Hu, P. and Rejtar, T. and Karger, B. L.},
   article_location = {Washington, D.C., USA},
   article_number = {20},
   keywords = {MALDI; CE; interface; matrix-assisted laser desorption; vacuum; deposition; peptides; proteins; proteome; proteomic; digest},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface},
   volume = {72},
   year = {2000}
}

TY  - JOUR
ID  - 357876
AU  - Preisler, J. - Hu, P. - Rejtar, T. - Karger, B. L.
PY  - 2000
TI  - Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface
JF  - Analytical Chemistry
VL  - 72
IS  - 20
SP  - 4785
EP  - 4785
PB  - American Chemical Society
SN  - 00032700
KW  - MALDI
KW  - CE
KW  - interface
KW  - matrix-assisted laser desorption
KW  - vacuum
KW  - deposition
KW  - peptides
KW  - proteins
KW  - proteome
KW  - proteomic
KW  - digest
N2  - An improved vacuum deposition interface for coupling capillary electrophoresis with MALDI/TOF MS has been developed. Liquid samples consisting of analyte and matrix were deposited on a moving tape in the evacuated source chamber of TOF mass spectrometer, enabling a day of continuous analysis without interruption. The vacuum deposition procedure was compared with the dried-droplet method, and it was found that vacuum deposition generated significantly more reproducible signal intensity, eliminating the need for the "sweet spot" searching. In addition, ion suppression caused by hydrophobicity differences in the analytes was reduced. In order to minimize ion suppression further, separation prior to MALDI/MS analysis was employed. The performance of capillary electrophoresis (CE) - MALDI/TOF MS using the vacuum deposition interface was evaluated with a peptide mixture injected at low femtomole levels. All peptides were baseline resolved with separation efficiencies in the range of 250,000 - 400,000 plates/m (2 - 3 second band halfwidth), demonstrating the high separation efficiency of the CE-MALDI/MS coupling.
ER  -

PREISLER, J., P. HU, T. REJTAR a B. L. KARGER. Capillary electrophoresis - Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using a vacuum deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2000, roč.~72, č.~20, s.~4785-4795. ISSN~0003-2700.

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