2001
Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.
VOTAVA, Miroslav, Renata TEJKALOVÁ, Marie DRÁBKOVÁ, Vít UNZEITIG, Ilja BRAVENY et. al.Základní údaje
Originální název
Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.
Název anglicky
Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor.
Autoři
VOTAVA, Miroslav, Renata TEJKALOVÁ, Marie DRÁBKOVÁ, Vít UNZEITIG a Ilja BRAVENY
Vydání
Europ. J. Clin. Microbiol. Infect. Dis. Heidelberg, Springer-Verlag, 2001, 0934-9723
Další údaje
Typ výsledku
Článek v odborném periodiku
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 1.386
Organizační jednotka
Lékařská fakulta
UT WoS
000167531600008
Klíčová slova anglicky
group B streptococci - rapid detection - women in labor - vaginal swabs - rectal swabs - GBS media - culture od GBS - naternal GBS colonization - neonatal GBS colonization
Změněno: 17. 6. 2009 15:20, prof. MUDr. Miroslav Votava, CSc.
V originále
In order to evaluate the differences in efficacy, three methods were used to detect GBS in women in labor. The recommended method for detecting GBS carriage in pregnant women is to culture vaginal and anorectal swabs in a selective broth medium and to subculture them onto blood agar. The method was compared with the use of GBS agar and GBS broth, both of which produce an orange pigment in response to GBS strains. A total of 319 women in labor were screened. Among the 638 specimens tested, 134 (21 %) were positive in the selective Todd-Hewitt broth subcultured onto sheep blood agar, 133 (20.8 %)were positive on the GBS agar and 126 (19.7 %) were positive in the GBS broth. Altogether, 89 (27.9 %) women in labor were found to be colonized with GBS; 87 (97.8 %) of them were identified as carriers using the Todd-Hewitt broth, 87 (97.8 %) with the GBS agar and 86 (96.6 %) with the GBS broth. These results indicate that both GBS agar and GBS broth are reliable methods that can be used to screen for maternat and neonatal GBS colonization.
Česky
In order to evaluate the differences in efficacy, three methods were used to detect GBS in women in labor. The recommended method for detecting GBS carriage in pregnant women is to culture vaginal and anorectal swabs in a selective broth medium and to subculture them onto blood agar. The method was compared with the use of GBS agar and GBS broth, both of which produce an orange pigment in response to GBS strains. A total of 319 women in labor were screened. Among the 638 specimens tested, 134 (21 %) were positive in the selective Todd-Hewitt broth subcultured onto sheep blood agar, 133 (20.8 %)were positive on the GBS agar and 126 (19.7 %) were positive in the GBS broth. Altogether, 89 (27.9 %) women in labor were found to be colonized with GBS; 87 (97.8 %) of them were identified as carriers using the Todd-Hewitt broth, 87 (97.8 %) with the GBS agar and 86 (96.6 %) with the GBS broth. These results indicate that both GBS agar and GBS broth are reliable methods that can be used to screen for maternat and neonatal GBS colonization.
Anglicky
In order to evaluate the differences in efficacy, three methods were used to detect GBS in women in labor. The recommended method for detecting GBS carriage in pregnant women is to culture vaginal and anorectal swabs in a selective broth medium and to subculture them onto blood agar. The method was compared with the use of GBS agar and GBS broth, both of which produce an orange pigment in response to GBS strains. A total of 319 women in labor were screened. Among the 638 specimens tested, 134 (21 %) were positive in the selective Todd-Hewitt broth subcultured onto sheep blood agar, 133 (20.8 %)were positive on the GBS agar and 126 (19.7 %) were positive in the GBS broth. Altogether, 89 (27.9 %) women in labor were found to be colonized with GBS; 87 (97.8 %) of them were identified as carriers using the Todd-Hewitt broth, 87 (97.8 %) with the GBS agar and 86 (96.6 %) with the GBS broth. These results indicate that both GBS agar and GBS broth are reliable methods that can be used to screen for maternat and neonatal GBS colonization.