1999
Effect of preoperative apheresis on the amount of apoptotic cells in peripheral blood of pigs during cardiopulmonary bypass
ŠPANOVÁ, Alena, Eva MATALOVÁ, Bohuslav RITTICH, Robert WAGNER, František KOVÁŘŮ et. al.Základní údaje
Originální název
Effect of preoperative apheresis on the amount of apoptotic cells in peripheral blood of pigs during cardiopulmonary bypass
Autoři
ŠPANOVÁ, Alena, Eva MATALOVÁ, Bohuslav RITTICH, Robert WAGNER a František KOVÁŘŮ
Vydání
Physiological Research, Praha, Institute of Physiology, CAS, CR, 1999, 0862-8408
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
Genetika a molekulární biologie
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 0.521
Kód RIV
RIV/00216224:14310/99:00003588
Organizační jednotka
Přírodovědecká fakulta
Změněno: 29. 3. 2001 10:57, doc. Ing. Bohuslav Rittich, CSc.
Anotace
V originále
Cardiopulmonary bypass (CPB) causes the postperfusion syndrom.The aim of our work was to show if the preoperative apheresis can lead to the decrease of apoptotic cells in blood. The presence of apoptotic cells was followed according to DNA isolated from granulocytes, lymfocytes and cells captured on apheretic filter. The experiment showed that apoptotic DNA was present in leucocytes of peripheral blood. However, the estimation of DNA fragmentation was negative in blood cells captured on the filter. It follows that preoperative apheresis can not markedly influence the amount of apioptotic cells in peripheral blood of pigs with CPB. Apoptosis was detected at the beginning of operation in cells of peripheral blood. The apoptotic response can be assumed in consequence of the stress situation.
Návaznosti
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