Application of Capillary Chromatography using Macroporous
Polyacrylamide Columns for the Analysis of Lignans from Seeds
of Schisandra chinensis

J 2001

Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Hana ŠTĚRBOVÁ, Vladislav KAHLE, Jiří SLANINA et. al.

Základní údaje

Originální název

Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis

Autoři

KVASNIČKOVÁ, Leona (203 Česká republika), Zdeněk GLATZ (203 Česká republika, garant), Hana ŠTĚRBOVÁ (203 Česká republika), Vladislav KAHLE, Jiří SLANINA (203 Česká republika) a Pavel MUSIL (203 Česká republika)

Vydání

Journal of Chromatography A, Netherlands, Elsevier, 2001, 0021-9606

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.147

Kód RIV

RIV/00216224:14310/01:00003598

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000168460400029

Klíčová slova anglicky

Capillary electrochromatography; Polymer columns; Lignans; Schizandra

Štítky

Capillary electrochromatography, lignans, Polymer columns, Schizandra

Změněno: 20. 6. 2009 00:24, Mgr. Jiří Slanina, Ph.D.

Anotace

V originále

Capillary electrochromatography using polymer based monolithic stationary phase has been developed as a promising method for the determination of lignans of Schisandra chinensis. The columns were prepared by in situ copolymerisation of acrylamide, N,N-methylene-bis-acrylamide, vinylsulfonic acid and lauryl acrylate in presence of poly(ethylene glycol) as a porogenic agent. The columns (75 ěm inner diameter; 33 cm total length; 24.5 cm effective length) were successfully used to analyse and quantify the lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 35 min. The calibration graphs were linear in the range 0.025 - 1.0 mg/ml of given lignan with correlation coefficients between 0.9951 and 0.9996. The inter-day reproducibility of the peak area were below 3.9 % and the inter-day reproducibility of the migration time were below 4.2 %.The results of quantitative CEC analyses were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by HPLC.

Návaznosti

GA303/00/D062, projekt VaV

Název: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Grantová agentura ČR, Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Citovat

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Hana ŠTĚRBOVÁ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL. Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis. Journal of Chromatography A. Netherlands: Elsevier, 2001, roč. 916, (1+2), s. 265-271. ISSN 0021-9606.

@article{359471,
   author = {Kvasničková, Leona and Glatz, Zdeněk and Štěrbová, Hana and Kahle, Vladislav and Slanina, Jiří and Musil, Pavel},
   article_location = {Netherlands},
   article_number = {(1+2)},
   keywords = {Capillary electrochromatography; Polymer columns; Lignans; Schizandra},
   language = {eng},
   issn = {0021-9606},
   journal = {Journal of Chromatography A},
   title = {Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis},
   volume = {916},
   year = {2001}
}

TY  - JOUR
ID  - 359471
AU  - Kvasničková, Leona - Glatz, Zdeněk - Štěrbová, Hana - Kahle, Vladislav - Slanina, Jiří - Musil, Pavel
PY  - 2001
TI  - Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis
JF  - Journal of Chromatography A
VL  - 916
IS  - (1+2)
SP  - 265
EP  - 265
PB  - Elsevier
SN  - 00219606
KW  - Capillary electrochromatography
KW  - Polymer columns
KW  - Lignans
KW  - Schizandra
N2  - Capillary electrochromatography using polymer based monolithic stationary phase has been developed as a promising method for the determination of lignans of Schisandra chinensis. The columns were prepared by in situ copolymerisation of acrylamide, N,N-methylene-bis-acrylamide, vinylsulfonic acid and lauryl acrylate in presence of poly(ethylene glycol) as a porogenic agent. The columns (75 ěm inner diameter; 33 cm total length; 24.5 cm effective length) were successfully used to analyse and quantify the lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 35 min. The calibration graphs were linear in the range 0.025 - 1.0 mg/ml of given lignan with correlation coefficients between 0.9951 and 0.9996. The inter-day reproducibility of the peak area were below 3.9 % and the inter-day reproducibility of the migration time were below 4.2 %.The results of quantitative CEC analyses were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in a good agreement with those determined by HPLC.
ER  -

KVASNIČKOVÁ, Leona, Zdeněk GLATZ, Hana ŠTĚRBOVÁ, Vladislav KAHLE, Jiří SLANINA a Pavel MUSIL. Application of Capillary Chromatography using Macroporous Polyacrylamide Columns for the Analysis of Lignans from Seeds of Schisandra chinensis. \textit{Journal of Chromatography A}. Netherlands: Elsevier, 2001, roč.~916, (1+2), s.~265-271. ISSN~0021-9606.

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