Stanovení biologicky aktivních látek v léčivých rostlinách
pomocí kapilární elektrochromatografie

D 2001

Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA et. al.

Basic information

Original name

Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie

Authors

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA and Pavel MUSIL

Edition

Brno, Sborník abstrakt 5. setkání biochemiků a molekulárních biologů, p. 47-48, 2001

Publisher

Přírodovědecká fakulta MU v Brně

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/01:00003599

Organization unit

Faculty of Science

ISBN

80-210-2538-7

Keywords in English

Medicinal Plants; Capillary electrochromatography

Abstract

V originále

Capillary electrochromatography (CEC) is a hybrid separation technique that combines the benefits of both capillary zone electrophoresis and high performance liquid chromatography. The high efficiency and resolution of CEC offers an alternative to pressure-driven chromatography systems. Polymer based monolithic capillary columns quickly become a well-established stationary phases in CEC as they eliminated the need of frits and packing procedures. The large variety of available chemistries make these columns an attractive alternative to columns packed with particulate materials. The macroporous polyacrylamide columns for CEC were prepared by in situ copolymerisation of acrylamide, bisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of polyethylene glycol as a porogenic agent. The columns were successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 20 min. The migration time and peak area reproducibilities were better than 4 %. The results were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in good agreement with those determined by HPLC.

Links

GA303/00/D062, research and development project

Name: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů

Investor: Czech Science Foundation, Bioavailability and mechanism of antioxidant activity of phenolic acids and lignans

Citovat

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA and Pavel MUSIL. Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie (Determination of Biologically Active Compounds in Medicinal Plants by Capillary Electrochromatography). In Sborník abstrakt 5. setkání biochemiků a molekulárních biologů. Brno: Přírodovědecká fakulta MU v Brně, 2001, p. 47-48. ISBN 80-210-2538-7.

@inproceedings{359473,
   author = {Kvasničková, Leona and Štěrbová, Hana and Glatz, Zdeněk and Kahle, Vladislav and Slanina, Jiří and Musil, Pavel},
   address = {Brno},
   booktitle = {Sborník abstrakt 5. setkání biochemiků a molekulárních biologů},
   keywords = {Medicinal Plants; Capillary electrochromatography},
   language = {eng},
   location = {Brno},
   isbn = {80-210-2538-7},
   pages = {47-48},
   publisher = {Přírodovědecká fakulta MU v Brně},
   title = {Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie},
   year = {2001}
}

TY  - JOUR
ID  - 359473
AU  - Kvasničková, Leona - Štěrbová, Hana - Glatz, Zdeněk - Kahle, Vladislav - Slanina, Jiří - Musil, Pavel
PY  - 2001
TI  - Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie
PB  - Přírodovědecká fakulta MU v Brně
CY  - Brno
SN  - 8021025387
KW  - Medicinal Plants
KW  - Capillary electrochromatography
N2  - Capillary electrochromatography (CEC) is a hybrid separation technique that combines the benefits of both capillary zone electrophoresis and high performance liquid chromatography. The high efficiency and resolution of CEC offers an alternative to pressure-driven chromatography systems. Polymer based monolithic capillary columns quickly become a well-established stationary phases in CEC as they eliminated the need of frits and packing procedures. The large variety of available chemistries make these columns an attractive alternative to columns packed with particulate materials. The macroporous polyacrylamide columns for CEC were prepared by in situ copolymerisation of acrylamide, bisacrylamide, vinylsulfonic acid and lauryl acrylate in presence of polyethylene glycol as a porogenic agent. The columns were successfully used to analyse and quantify lignans in extract of the seeds of Schisandra chinensis. Good separations were achieved in less than 20 min. The migration time and peak area reproducibilities were better than 4 %. The results were compared with those obtained by reverse-phase HPLC, the levels of schizandrin, gomisin A, gomisin N and wuweizisu C determined by CEC were in good agreement with those determined by HPLC.
ER  -

KVASNIČKOVÁ, Leona, Hana ŠTĚRBOVÁ, Zdeněk GLATZ, Vladislav KAHLE, Jiří SLANINA and Pavel MUSIL. Stanovení biologicky aktivních látek v léčivých rostlinách pomocí kapilární elektrochromatografie (Determination of Biologically Active Compounds in Medicinal Plants by Capillary Electrochromatography). In \textit{Sborník abstrakt 5. setkání biochemiků a molekulárních biologů}. Brno: Přírodovědecká fakulta MU v Brně, 2001, p.~47-48. ISBN~80-210-2538-7.

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