2001
Exchange aberrations among 11 chromosomes of human lymphocytes induced by gamma-rays
GAŇOVÁ, Alena, Emilie LUKÁŠOVÁ, Stanislav KOZUBEK, Michal KOZUBEK, Raisa GOVORUN et. al.Základní údaje
Originální název
Exchange aberrations among 11 chromosomes of human lymphocytes induced by gamma-rays
Autoři
GAŇOVÁ, Alena (203 Česká republika), Emilie LUKÁŠOVÁ (203 Česká republika), Stanislav KOZUBEK (203 Česká republika), Michal KOZUBEK (203 Česká republika), Raisa GOVORUN, Irena KOUTNÁ (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Magdalena SKALNÍKOVÁ (203 Česká republika), Pavla JIRSOVÁ (203 Česká republika), Renata PASEKOVÁ (203 Česká republika) a Evgenij KRASAVIN
Vydání
International Journal of Radiation Biology, 2001, 0955-3002
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10610 Biophysics
Stát vydavatele
Velká Británie a Severní Irsko
Utajení
není předmětem státního či obchodního tajemství
Odkazy
Impakt faktor
Impact factor: 2.356
Kód RIV
RIV/00216224:14330/01:00004206
Organizační jednotka
Fakulta informatiky
UT WoS
000167998200003
Klíčová slova anglicky
INDUCED STRUCTURAL-ABERRATIONS; INDUCED CHROMATIN BREAKS; IN-SITU HYBRIDIZATION; DOUBLE-STRAND BREAKS; IONIZING-RADIATION; CELL-NUCLEI; DOMAINS; TRANSLOCATIONS; REPAIR; ORGANIZATION
Štítky
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 7. 5. 2010 16:34, prof. RNDr. Michal Kozubek, Ph.D.
Anotace
V originále
Purpose: To detect the frequencies of inter-changes among 11 chromosomes in lymphocytes irradiated with gamma -rays and to find out whether these frequencies reflect the proximity of some of these chromosomes within the interphase nucleus. Material and methods: Exchange aberrations were detected in the first mitosis after irradiation of human lymphocytes with 3 and 5 Gy gamma -rays of` Co-60. Two-colour repeated FISH with two differently chemically modified probes in each hybridization was applied. The microscope stage positions of each mitosis were recorded after the first hybridization and used for the automatic scanning of images after all successive experiments. Five images were obtained for each mitosis differing in visualized pairs of chromosomes. Comparing these images, exchanges among 10 chromosomes could be detected. Painting of the p arm of chromosome 21 with the painting probe for chromosome 22 also made it possible to detect exchanges of this chromosome with other chromosomes of the selected group. Results: Frequencies of exchange aberrations induced in chromosomes of the selected group as well as interchanges between many pairs of chromosomes of this group were roughly proportional to the DNA content of chromosomes. Higher frequencies of interchanges than expected according to the model of linear proportionality were found between several chromosomes involved in translocations frequent in different subtypes of leukaemia. Conclusions: Frequencies of interchanges among 11 chromosomes of human lymphocytes induced by gamma -rays do not indicate as clearly as fast neutrons the non-random arrangement of chromosomes in the cell nucleus. The interaction of a large number of chromosomes in exchange aberrations suggests that the chromatirl in the territory of one chromosome is accessible for several other chromosomes.
Návaznosti
| GA202/01/0197, projekt VaV |
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| GA524/98/0190, projekt VaV |
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| IBS5004010, projekt VaV |
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| MSM 143300002, záměr |
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| NC5955, projekt VaV |
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| NM15, projekt VaV |
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