2001
Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex
HOFR, Ctirad, Nicholas FARRELL a Viktor BRABECZákladní údaje
Originální název
Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex
Autoři
HOFR, Ctirad, Nicholas FARRELL a Viktor BRABEC
Vydání
Nucleic Acids Research, Oxford, UK, Oxford Press, 2001, 0305-1048
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10610 Biophysics
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 6.373
Kód RIV
RIV/00216224:14310/01:00004214
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
DNA; thermodynamic stability; platinum drugs; calorimetry
Štítky
Změněno: 25. 5. 2001 15:21, prof. RNDr. Viktor Brabec, DrSc.
Anotace
V originále
Bifunctional polynuclear platinum compounds represent a novel class of metal-based antitumor drugs. A typical agent is [{trans-PtCl(NH3)2}2H2N(CH2)4NH2]Cl2, (1,1/t,t) which coordinates to bases in DNA and forms covalent cross-links. It also forms a 1,2-d(GpG) intrastrand adduct, the equivalent of the major DNA lesion of "classical" cisplatin. In the present study, differential scanning calorimetry and spectroscopic techniques were employed to characterize the influence of this cross-link on the thermal stability and energetics of 20 bp DNA duplexes site-specifically modified by 1,1/t,t. Thermal denaturation data revealed that the cross-link of 1,1/t,t reduced thermal and thermodynamical stability of the duplex noticeably more than that of "classical" cisplatin. The energetic consequences of the intrastrand cross-link at the d(GG) site are discussed in relation to the structural distortions induced by this adduct in DNA and to its recognition and binding by HMG-domain proteins.
Návaznosti
| GA305/99/0695, projekt VaV |
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