CHROUST, Karel, Trevor JOWETT, Mustafa FARID-WAJIDI, Juan HUANG, Martina RYŠKOVÁ, Ronald WOLF a Ivan HOLOUBEK. Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. Mutation Research. Amsterdam: Elsevier, 2000, roč. 2001, 498(1-2), s. 169-179. ISSN 0165-1218. |
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@article{371654, author = {Chroust, Karel and Jowett, Trevor and FaridandWajidi, Mustafa and Huang, Juan and Ryšková, Martina and Wolf, Ronald and Holoubek, Ivan}, article_location = {Amsterdam}, article_number = {498(1-2)}, keywords = {Transgenic Drosophila melanogaster; wing spot test; female sterility; aneuploidy; glutathione S-transferase; 1;2:5;6-dibenzanthracene; 1;2-dichloroethane}, language = {eng}, issn = {0165-1218}, journal = {Mutation Research}, title = {Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase.}, volume = {2001}, year = {2000} }
TY - JOUR ID - 371654 AU - Chroust, Karel - Jowett, Trevor - Farid-Wajidi, Mustafa - Huang, Juan - Ryšková, Martina - Wolf, Ronald - Holoubek, Ivan PY - 2000 TI - Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. JF - Mutation Research VL - 2001 IS - 498(1-2) SP - 169 EP - 169 PB - Elsevier SN - 01651218 KW - Transgenic Drosophila melanogaster KW - wing spot test KW - female sterility KW - aneuploidy KW - glutathione S-transferase KW - 1;2:5;6-dibenzanthracene KW - 1;2-dichloroethane N2 - Sensitivity of transgenic Drosophila melanogaster with expression of a human gene encoding the glutathione S-transferase alpha subunit (GSTA1-1) to 1,2:5,6-dibenzanthracene (DBA) and 1,2-dichloroethane (DCE) was investigated in the somatic mutation and recombination test (SMART). We performed the same assay in control transgenic flies expressing the bacterial lacZ gene. Three types of transgenic Drosophila strains carrying GSTA1-1 were used: two transgenic strains homozygous for the second chromosome with a single-copy transgene insertion and one strain with two transgene insertions. Larvae carrying the lacZ gene were significantly more sensitive to genotoxic effects of DBA than those carrying three copies of the GSTA1-1 gene. The larvae with lacZ expression showed significantly lower sensitivity to DCE compared with those expressing GSTA1-1. Finally, a pretreatment with buthionine-sulphoximine (BSO) in experiment with DCE significantly decreased the frequency of mutation events in larvae with three GSTA1-1 copies in comparison with others. ER -
CHROUST, Karel, Trevor JOWETT, Mustafa FARID-WAJIDI, Juan HUANG, Martina RYŠKOVÁ, Ronald WOLF a Ivan HOLOUBEK. Activation or detoxification of mutagenic and carcinogenic compounds in transgenic Drosophila expressing human glutathione S-transferase. \textit{Mutation Research}. Amsterdam: Elsevier, 2000, roč.~2001, 498(1-2), s.~169-179. ISSN~0165-1218.
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