A proposal of new indices for soil microbial ecotoxicology

D 2001

A proposal of new indices for soil microbial ecotoxicology

HOFMAN, Jakub, Ladislav DUŠEK a Ludvík DOLEŽAL

Základní údaje

Originální název

A proposal of new indices for soil microbial ecotoxicology

Autoři

HOFMAN, Jakub, Ladislav DUŠEK a Ludvík DOLEŽAL

Vydání

Brussels, Belgium, Abstracts of SETAC 2001: 11th Annual Meeting of SETAC Europe; 6-10 May 2001, Madrid, Spain, s. 74-364, 2001

Nakladatel

SETAC Europe

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00004625

Organizační jednotka

Přírodovědecká fakulta

Změněno: 12. 11. 2001 15:29, prof. RNDr. Jakub Hofman, Ph.D.

Anotace

V originále

A classical chloroform fumigation-extraction method (CFEM) was carried out to obtain 0.5M K2SO4 extracts of fumigated (F) and non-fumigated (NF) samples of 36 grassland and 12 forest soils from Czech Republic. Main physical and chemical properties of soils were also determined and basal respiration (BR) of soils was measured. Organic C in extracts was determined by dichromate digestion. Anthrone-, orcinol-, and phenol-reactive C (ARC, ORC, and PRC, respectively), ninhydrin-reactive N (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC), and diphenylamine-reactive compounds (DRC) were assessed in extracts using colorimetric analytical methods. The methods used were found to be quick, simple and cheap. By this way biochemical specification of soil organic matter and microbial biomass was gained. Results of measurements were proposed to make several types of indices. The index expressions of measured parameters were found to be the most suitable for (a) evaluation and investigation of relationships between microbial biomass and soil organic matter, and for (b) soil organic matter composition studies. Three main types of indices were proposed: index I. as extracellular amount of each compound to microbial biomass, index II., "immobilization ratio", as proportion of each compound between soil extracellular organic matter and microbial biomass, and index III., "substrate quality index", as extracellular amount of each compound to extractable extracellular carbon (EX-C). The possibility of characterizing soil organic matter and microbial biomass C and N compounds by adopting suitable colorimetric methods is another practical advantage of CFEM and could show a possible course of improvement in soil microbial ecotoxicology.

Návaznosti

GA526/98/1147, projekt VaV

Název: Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)

Investor: Grantová agentura ČR, Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)

SI/340/1/00, projekt VaV

Název: Region specifický přístup v analýze ekologických rizik - metodika zhodnocení ve vztahu k přírodním katastrofám.

Citovat

HOFMAN, Jakub, Ladislav DUŠEK a Ludvík DOLEŽAL. A proposal of new indices for soil microbial ecotoxicology. In Abstracts of SETAC 2001: 11th Annual Meeting of SETAC Europe; 6-10 May 2001, Madrid, Spain. Brussels, Belgium: SETAC Europe, 2001, s. 74-364.

@inproceedings{374931,
   author = {Hofman, Jakub and Dušek, Ladislav and Doležal, Ludvík},
   address = {Brussels, Belgium},
   booktitle = {Abstracts of SETAC 2001: 11th Annual Meeting of SETAC Europe; 6-10 May 2001, Madrid, Spain},
   language = {eng},
   location = {Brussels, Belgium},
   pages = {74-364},
   publisher = {SETAC Europe},
   title = {A proposal of new indices for soil microbial ecotoxicology},
   year = {2001}
}

TY  - JOUR
ID  - 374931
AU  - Hofman, Jakub - Dušek, Ladislav - Doležal, Ludvík
PY  - 2001
TI  - A proposal of new indices for soil microbial ecotoxicology
PB  - SETAC Europe
CY  - Brussels, Belgium
N2  - A classical chloroform fumigation-extraction method (CFEM) was carried out to obtain 0.5M K2SO4 extracts of fumigated (F) and non-fumigated (NF) samples of 36 grassland and 12 forest soils from Czech Republic. Main physical and chemical properties of soils were also determined and basal respiration (BR) of soils was measured. Organic C in extracts was determined by dichromate digestion. Anthrone-, orcinol-, and phenol-reactive C (ARC, ORC, and PRC, respectively), ninhydrin-reactive N (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC), and diphenylamine-reactive compounds (DRC) were assessed in extracts using colorimetric analytical methods. The methods used were found to be quick, simple and cheap. By this way biochemical specification of soil organic matter and microbial biomass was gained. Results of measurements were proposed to make several types of indices. The index expressions of measured parameters were found to be the most suitable for (a) evaluation and investigation of relationships between microbial biomass and soil organic matter, and for (b) soil organic matter composition studies. Three main types of indices were proposed: index I. as extracellular amount of each compound to microbial biomass, index II., "immobilization ratio", as proportion of each compound between soil extracellular organic matter and microbial biomass, and index III., "substrate quality index", as extracellular amount of each compound to extractable extracellular carbon (EX-C). The possibility of characterizing soil organic matter and microbial biomass C and N compounds by adopting suitable colorimetric methods is another practical advantage of CFEM and could show a possible course of improvement in soil microbial ecotoxicology.
ER  -

HOFMAN, Jakub, Ladislav DUŠEK a Ludvík DOLEŽAL. A proposal of new indices for soil microbial ecotoxicology. In \textit{Abstracts of SETAC 2001: 11th Annual Meeting of SETAC Europe; 6-10 May 2001, Madrid, Spain}. Brussels, Belgium: SETAC Europe, 2001, s.~74-364.

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