Methodical progress of microbial biomass determination with
classical fumigation-extraction method - further possible
improvement in soil microbial ecotoxicology

D 2000

Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology

HOFMAN, Jakub

Základní údaje

Originální název

Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology

Autoři

HOFMAN, Jakub

Vydání

Praha, Symposium Program of Prague 2000: Fifth International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe; 12-14 September 2000, Prague, Czech Republic, s. 144-455, 2000

Nakladatel

Institute for International Cooperative Environmental Research

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/00:00004626

Organizační jednotka

Přírodovědecká fakulta

Změněno: 12. 11. 2001 15:39, prof. RNDr. Jakub Hofman, Ph.D.

Anotace

V originále

Fumigation-extraction method (FEM) is one of the main methodical implements of current soil microbiology. FEM serves to quantify microbial biomass as carbon immobilized in microorganisms - Cbio. Principle of the method is dispatching of soil microorganism cells with fumigant (redestilled chloroform). Biomass is obtained from 0.5M K2SO4 extractable carbon difference from fumigated and non-fumigated samples. Study was focused on biochemical specification of soil organic matter extracted 0.5M K2SO4 before and after fumigation. Colorimetric analytical methods were found to be the most suitable alternative for this purpose. The organic C (Cox), phenol-, anthrone- and orcinol-reactive organic compounds (PRC, ARC and ORC), ninhydrin-reactive nitrogen (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC) and diphenylamine-reactive compounds (DRC) were evaluated using extracts from 48 different soils. All of the used methods were quick, simple and cheap. Parameters obtained from such measurements for fumigated and non-fumigated extracts were found to be good approach for FE method validation. Index expressions of measured parameters were found as the most suitable for evaluation and investigation of relationships between microbial biomass and soil organic matter. Indices were found to be sensitive indicators for soil matter composition and microbial biomass mineralization activity changes. Furthermore, indices discriminated measured soils more sensitively than Cbio measures only. The possibility of characterizing soil organic matter and microbial biomass by adopting suitable colorimetric methods is another advantage of fumigation-extraction method and could show a possible course of improvement in soil microbial ecotoxicology.

Návaznosti

GA526/98/1147, projekt VaV

Název: Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)

Investor: Grantová agentura ČR, Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)

Citovat

HOFMAN, Jakub. Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology. In Symposium Program of Prague 2000: Fifth International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe; 12-14 September 2000, Prague, Czech Republic. Praha: Institute for International Cooperative Environmental Research, 2000, s. 144-455.

@inproceedings{374935,
   author = {Hofman, Jakub},
   address = {Praha},
   booktitle = {Symposium Program of Prague 2000: Fifth International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe; 12-14 September 2000, Prague, Czech Republic},
   language = {eng},
   location = {Praha},
   pages = {144-455},
   publisher = {Institute for International Cooperative Environmental Research},
   title = {Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology},
   year = {2000}
}

TY  - JOUR
ID  - 374935
AU  - Hofman, Jakub
PY  - 2000
TI  - Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology
PB  - Institute for International Cooperative Environmental Research
CY  - Praha
N2  - Fumigation-extraction method (FEM) is one of the main methodical implements of current soil microbiology. FEM serves to quantify microbial biomass as carbon immobilized in microorganisms - Cbio. Principle of the method is dispatching of soil microorganism cells with fumigant (redestilled chloroform). Biomass is obtained from 0.5M K2SO4 extractable carbon difference from fumigated and non-fumigated samples. Study was focused on biochemical specification of soil organic matter extracted 0.5M K2SO4 before and after fumigation. Colorimetric analytical methods were found to be the most suitable alternative for this purpose. The organic C (Cox), phenol-, anthrone- and orcinol-reactive organic compounds (PRC, ARC and ORC), ninhydrin-reactive nitrogen (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC) and diphenylamine-reactive compounds (DRC) were evaluated using extracts from 48 different soils. All of the used methods were quick, simple and cheap. Parameters obtained from such measurements for fumigated and non-fumigated extracts were found to be good approach for FE method validation. Index expressions of measured parameters were found as the most suitable for evaluation and investigation of relationships between microbial biomass and soil organic matter. Indices were found to be sensitive indicators for soil matter composition and microbial biomass mineralization activity changes. Furthermore, indices discriminated measured soils more sensitively than Cbio measures only. The possibility of characterizing soil organic matter and microbial biomass by adopting suitable colorimetric methods is another advantage of fumigation-extraction method and could show a possible course of improvement in soil microbial ecotoxicology.
ER  -

HOFMAN, Jakub. Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology. In \textit{Symposium Program of Prague 2000: Fifth International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe; 12-14 September 2000, Prague, Czech Republic}. Praha: Institute for International Cooperative Environmental Research, 2000, s.~144-455.

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