Detailed Information on Publication Record
2001
Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)
ZURAWSKI, Tomáš, A. MOUSLEY, G.R. MAIR, G.P. BRENNAN, A.G. MAULE et. al.Basic information
Original name
Immunomicroscopical observation on the nervous system of adult Eudiplozoon nippnicum (Monogenea: Diplozoidae)
Authors
ZURAWSKI, Tomáš, A. MOUSLEY, G.R. MAIR, G.P. BRENNAN, A.G. MAULE, Milan GELNAR and D.W. HALTON
Edition
International Journal for Parasitology, Australia, Elsevier, 2001, 0020-7519
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.814
RIV identification code
RIV/00216224:14310/01:00004774
Organization unit
Faculty of Science
Keywords in English
monogenean neurobiology; cholinesterase; serotonin; neuropeptides; confocal microscopy; immunogold labeling
Tags
Změněno: 28/11/2001 18:36, prof. RNDr. Milan Gelnar, CSc.
Abstract
V originále
Neuronal pathways have been examined in adult Eudiplozoon nipponicum (Monogenea: Diplozoidae) using cytochemistry interfaced with confocal scanning laser microscopy, in an attempt to ascertain the status of the nervous system. Peptidergic and serotinergic innervation was demonstrated by indirect immunocytochemistry and cholinnergic componets by enzyme cytochemical methodology; post-embeding electron microscopical immunogold labelling revealed neuropeptide immunoreactivity at the subcellular level. All three classes of neuronal mediators were identified throught both central and peripheral elements of a well-differentiated orthogonal nervous systems.There was considerable overlap in the staining patterns for cholinergic and peptinergic compoments; while dual immunostaining revealed serotonin immunoreactivity to be largely confined to a separate set of neurons. The subcellular distribution of immunoreactivity to the flatworm neuropeptide, GYIRFamide, confirmed neuropeptide localisation in dense-cored vesicles in the majority of the axons and terminal varicosities of both central and peripheral nervous systems. Result revealed an extensive and chemically diverse nervous system and suggest that pairing of individuals involves fusion of central nerve elements; it is likely also that there is continuity between the peripheral nervous systems of the two partner worms.
Links
GA524/00/0844, research and development project |
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MSM 143100010, plan (intention) |
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