PCR identification of Salmonella cells in food and stool
samples after immunomagnetic separation

J 2001

PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation

ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ, Denisa ŠKAPOVÁ et. al.

Základní údaje

Originální název

PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation

Autoři

ŠPANOVÁ, Alena (203 Česká republika, garant), Bohuslav RITTICH (203 Česká republika), Renata KARPÍŠKOVÁ, Leona ČECHOVÁ (203 Česká republika) a Denisa ŠKAPOVÁ (203 Česká republika)

Vydání

Bioseparation, Nizozemsko, Kluwer Academic Publishers, 2001, 0923-179X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.054

Kód RIV

RIV/00216224:14310/01:00004808

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000169737400006

Klíčová slova anglicky

Salmonella; PCR; IMS; inhibitors; food and stool samples

Štítky

food and stool samples, IMS, inhibitors, PCR, Salmonella

Změněno: 13. 11. 2006 17:50, doc. Ing. Bohuslav Rittich, CSc.

Anotace

V originále

The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure.

Návaznosti

GA203/98/1231, projekt VaV

Název: Orientovaná imobilizace enzymů a protilátek s využitím biospecifických interakcí

Investor: Grantová agentura ČR, Orientovaná imobilizace enzymů a protilátek s využitím biospecifických interakcí

IZ3988, projekt VaV

Název: Vývoj a ověření imunologických a molekulárně genetických metod k detekci vybraných patogenů z potravinového řetězce

Citovat

ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ a Denisa ŠKAPOVÁ. PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation. Bioseparation. Nizozemsko: Kluwer Academic Publishers, 2001, roč. 9, č. 6, s. 379-384. ISSN 0923-179X.

@article{378501,
   author = {Španová, Alena and Rittich, Bohuslav and Karpíšková, Renata and Čechová, Leona and Škapová, Denisa},
   article_location = {Nizozemsko},
   article_number = {6},
   keywords = {Salmonella; PCR; IMS; inhibitors; food and stool samples},
   language = {eng},
   issn = {0923-179X},
   journal = {Bioseparation},
   title = {PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation},
   volume = {9},
   year = {2001}
}

TY  - JOUR
ID  - 378501
AU  - Španová, Alena - Rittich, Bohuslav - Karpíšková, Renata - Čechová, Leona - Škapová, Denisa
PY  - 2001
TI  - PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation
JF  - Bioseparation
VL  - 9
IS  - 6
SP  - 379
EP  - 379
PB  - Kluwer Academic Publishers
SN  - 0923179X
KW  - Salmonella
KW  - PCR
KW  - IMS
KW  - inhibitors
KW  - food and stool samples
N2  - The purpose of the present study was to investigate the application of various sample preparation methods (cell washing before lysis, purification of DNA using phenol extraction method, immunomagnetic separation-IMS) for the final PCR identification of Salmonella cells. The presence of PCR inhibitors in processed food products (milk powder and dried eggs) can be the cause of false-negative results in PCR without IMS of target cells. It was also demonstrated that IMS-PCR was succesfully used for identification and quick confirmation of untypical Salmonella strains isolated from human stool samples and rabbit meat. However, IMS cannot eliminate intracellular PCR inhibitors present in immunoseparated Salmonella cells. These inhibitors must be taken into consideration in evaluation of PCR procedure.
ER  -

ŠPANOVÁ, Alena, Bohuslav RITTICH, Renata KARPÍŠKOVÁ, Leona ČECHOVÁ a Denisa ŠKAPOVÁ. PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation. \textit{Bioseparation}. Nizozemsko: Kluwer Academic Publishers, 2001, roč.~9, č.~6, s.~379-384. ISSN~0923-179X.

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