| HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD a Milan J. BENEŠ. Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres. Biotechnology Progress. U.S.A.: Am.Chem. Soc., Washington, 2001, roč. 17, č. 3, s. 447-452. ISSN 8756-7938. |
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@article{378510,
author = {Horák, Daniel and Rittich, Bohuslav and Šafář, Jan and Španová, Alena and Lenfeld, Jiří and Beneš, Milan J.},
article_location = {U.S.A.},
article_number = {3},
keywords = {Dispersion polymerization; RNase A; nucleic acids; particles; polystyrene; chromatography; purification},
language = {eng},
issn = {8756-7938},
journal = {Biotechnology Progress},
title = {Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres},
volume = {17},
year = {2001}
}
TY - JOUR
ID - 378510
AU - Horák, Daniel - Rittich, Bohuslav - Šafář, Jan - Španová, Alena - Lenfeld, Jiří - Beneš, Milan J.
PY - 2001
TI - Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres
JF - Biotechnology Progress
VL - 17
IS - 3
SP - 447
EP - 447
PB - Am.Chem. Soc., Washington
SN - 87567938
KW - Dispersion polymerization
KW - RNase A
KW - nucleic acids
KW - particles
KW - polystyrene
KW - chromatography
KW - purification
N2 - Magnetic hydrogel microsheres were prepared by dispersion copolymerisation of 2-hydroxyethyl methacrylate and ethylene dimethacrylate in the presence of magnetite, which formed the core of the particles. RNase A was coupled to the particles by the cyanuric chloride method. Gel electrophoresis of plasmid DNA pUC19 (contaminated by bacterial RNA) confirmed RNA degradation with the immobilized enzyme. The effect of temperature and pH on the relative activity of immobilized RNase A was estimated after incubation of the samples at different temperatures (30-80 C) and pH (4.0-8.0). Maximum relative activity was observed at 70 C and pH 6.5. The matrice based on magnetic poly(HEMA) had a low tendency to adsorb RNA.
ER -
HORÁK, Daniel, Bohuslav RITTICH, Jan ŠAFÁŘ, Alena ŠPANOVÁ, Jiří LENFELD a Milan J. BENEŠ. Properties of RNase A immobilized on magnetic poly(2-hydroxyethyl methacrylate) microspheres. \textit{Biotechnology Progress}. U.S.A.: Am.Chem. Soc., Washington, 2001, roč.~17, č.~3, s.~447-452. ISSN~8756-7938.
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