Influence of metal ions on activity and stability of DNase I
immobilized on hydrophilic particles

D 2001

Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles

RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK et. al.

Základní údaje

Originální název

Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles

Autoři

RITTICH, Bohuslav (203 Česká republika, garant), Alena ŠPANOVÁ (203 Česká republika), Yuriy OHLASHENNYY, Ivo RUDOLF (203 Česká republika), Daniel HORÁK, Jiří LENFELD a Milan J. BENEŠ

Vydání

2001. vyd. Zagreb, Croatia, The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists, s. 63-64, 2001

Nakladatel

Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Chorvatsko

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00004818

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

immobilization; DNase I; activity; stability; applications

Štítky

activity, applications, DNase I, immobilization, stability

Změněno: 13. 11. 2006 17:49, doc. Ing. Bohuslav Rittich, CSc.

Anotace

V originále

The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.

Návaznosti

GA203/00/1339, projekt VaV

Název: Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Investor: Grantová agentura ČR, Disperzní polymerizace hydrofilních monometrů pro přípravu magnetických nosičů používaných v biotechnologických aplikacích

Citovat

RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD a Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists. 2001. vyd. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, s. 63-64.

@inproceedings{378552,
   author = {Rittich, Bohuslav and Španová, Alena and Ohlashennyy, Yuriy and Rudolf, Ivo and Horák, Daniel and Lenfeld, Jiří and Beneš, Milan J.},
   address = {Zagreb, Croatia},
   booktitle = {The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists},
   edition = {2001},
   keywords = {immobilization; DNase I; activity; stability; applications},
   language = {eng},
   location = {Zagreb, Croatia},
   pages = {63-64},
   publisher = {Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia},
   title = {Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles},
   year = {2001}
}

TY  - JOUR
ID  - 378552
AU  - Rittich, Bohuslav - Španová, Alena - Ohlashennyy, Yuriy - Rudolf, Ivo - Horák, Daniel - Lenfeld, Jiří - Beneš, Milan J.
PY  - 2001
TI  - Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles
PB  - Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
CY  - Zagreb, Croatia
KW  - immobilization
KW  - DNase I
KW  - activity
KW  - stability
KW  - applications
N2  - The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.
ER  -

RITTICH, Bohuslav, Alena ŠPANOVÁ, Yuriy OHLASHENNYY, Ivo RUDOLF, Daniel HORÁK, Jiří LENFELD a Milan J. BENEŠ. Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles. In \textit{The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists}. 2001. vyd. Zagreb, Croatia: Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia, 2001, s.~63-64.

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