Investigation of highly sensitive piezoelectric immunosensors
for 2,4-dichlorophenoxyacetic acid

J 2001

Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid

HALÁMEK, Jan, Maria HEPEL a Petr SKLÁDAL

Základní údaje

Originální název

Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid

Autoři

HALÁMEK, Jan, Maria HEPEL a Petr SKLÁDAL

Vydání

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2001, 0956-5663

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.714

Kód RIV

RIV/00216224:14310/01:00004853

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000169712700005

Klíčová slova anglicky

quartz crystal microbalance; immunosensor; 2;4-dichlorophenoxyacetic acid

Štítky

2, 4-dichlorophenoxyacetic acid, immunosensor, Quartz crystal microbalance

Změněno: 5. 12. 2001 10:35, prof. RNDr. Petr Skládal, CSc.

Anotace

V originále

The improved highly sensitive piezoelectric immunosensor has been developed and evaluated using a model interaction of antibody with the model hapten-herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of cystamine, 4-aminothiophenol or 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) were formed on smooth and rough crystals coated with gold or silver electrodes. The immunochemical interactions performed well in all cases, the aminothiophenol on gold was chosen as the optimum with regard to regeneration of immunosensing surfaces. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. Therefore. the smooth crystal should be preferred for future kinetic studies. The competitive assay of the herbicide 2,4-D achieved a limit of detection of 10 ng/l using the monoclonal anti-2,4-D antibody F6C10. Finally, a direct assay format has been evaluated using a thicker layer of glutaraldehyde-crosslinked antibody on the sensing surface. The direct binding of a small herbicide molecule was follon ed in real time. The detected concentration of 2,4-D (5 ug/l) was low enough for future direct monitoring of this herbicide in water.

Návaznosti

OC 518.30, projekt VaV

Název: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů

Citovat

HALÁMEK, Jan, Maria HEPEL a Petr SKLÁDAL. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2001, roč. 16, č. 3, s. 253-260. ISSN 0956-5663.

@article{378954,
   author = {Halámek, Jan and Hepel, Maria and Skládal, Petr},
   article_location = {Oxford},
   article_number = {3},
   keywords = {quartz crystal microbalance; immunosensor; 2;4-dichlorophenoxyacetic acid},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid},
   volume = {16},
   year = {2001}
}

TY  - JOUR
ID  - 378954
AU  - Halámek, Jan - Hepel, Maria - Skládal, Petr
PY  - 2001
TI  - Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid
JF  - Biosensors & Bioelectronics
VL  - 16
IS  - 3
SP  - 253
EP  - 253
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - quartz crystal microbalance
KW  - immunosensor
KW  - 2;4-dichlorophenoxyacetic acid
N2  - The improved highly sensitive piezoelectric immunosensor has been developed and evaluated using a model interaction of antibody with the model hapten-herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of cystamine, 4-aminothiophenol or 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) were formed on smooth and rough crystals coated with gold or silver electrodes. The immunochemical interactions performed well in all cases, the aminothiophenol on gold was chosen as the optimum with regard to regeneration of immunosensing surfaces. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. Therefore. the smooth crystal should be preferred for future kinetic studies. The competitive assay of the herbicide 2,4-D achieved a limit of detection of 10 ng/l using the monoclonal anti-2,4-D antibody F6C10. Finally, a direct assay format has been evaluated using a thicker layer of glutaraldehyde-crosslinked antibody on the sensing surface. The direct binding of a small herbicide molecule was follon ed in real time. The detected concentration of 2,4-D (5 ug/l) was low enough for future direct monitoring of this herbicide in water.
ER  -

HALÁMEK, Jan, Maria HEPEL a Petr SKLÁDAL. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 2001, roč.~16, č.~3, s.~253-260. ISSN~0956-5663.

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