Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid
|HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2001, vol. 16, No 3, p. 253-260. ISSN 0956-5663.
|Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid
|HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL.
|Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2001, 0956-5663.
|Type of outcome
|Article in a journal
|Field of Study
|10600 1.6 Biological sciences
|Country of publisher
|United Kingdom of Great Britain and Northern Ireland
|is not subject to a state or trade secret
|Impact factor: 2.714
|RIV identification code
|Faculty of Science
|Keywords in English
|quartz crystal microbalance; immunosensor; 2;4-dichlorophenoxyacetic acid
|2, 4-dichlorophenoxyacetic acid, immunosensor, Quartz crystal microbalance
|Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 5/12/2001 10:35.
|The improved highly sensitive piezoelectric immunosensor has been developed and evaluated using a model interaction of antibody with the model hapten-herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of cystamine, 4-aminothiophenol or 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) were formed on smooth and rough crystals coated with gold or silver electrodes. The immunochemical interactions performed well in all cases, the aminothiophenol on gold was chosen as the optimum with regard to regeneration of immunosensing surfaces. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. Therefore. the smooth crystal should be preferred for future kinetic studies. The competitive assay of the herbicide 2,4-D achieved a limit of detection of 10 ng/l using the monoclonal anti-2,4-D antibody F6C10. Finally, a direct assay format has been evaluated using a thicker layer of glutaraldehyde-crosslinked antibody on the sensing surface. The direct binding of a small herbicide molecule was follon ed in real time. The detected concentration of 2,4-D (5 ug/l) was low enough for future direct monitoring of this herbicide in water.
|OC 518.30, research and development project
|Name: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů
|Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors
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