HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL. Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2001, vol. 16, No 3, p. 253-260. ISSN 0956-5663.
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Basic information
Original name Investigation of highly sensitive piezoelectric immunosensors for 2,4-dichlorophenoxyacetic acid
Authors HALÁMEK, Jan, Maria HEPEL and Petr SKLÁDAL.
Edition Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2001, 0956-5663.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 2.714
RIV identification code RIV/00216224:14310/01:00004853
Organization unit Faculty of Science
UT WoS 000169712700005
Keywords in English quartz crystal microbalance; immunosensor; 2;4-dichlorophenoxyacetic acid
Tags 2, 4-dichlorophenoxyacetic acid, immunosensor, Quartz crystal microbalance
Changed by Changed by: prof. RNDr. Petr Skládal, CSc., učo 2202. Changed: 5/12/2001 10:35.
The improved highly sensitive piezoelectric immunosensor has been developed and evaluated using a model interaction of antibody with the model hapten-herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). For immobilization of 2,4-D, the self-assembled layers of cystamine, 4-aminothiophenol or 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) were formed on smooth and rough crystals coated with gold or silver electrodes. The immunochemical interactions performed well in all cases, the aminothiophenol on gold was chosen as the optimum with regard to regeneration of immunosensing surfaces. The kinetics of interaction of surface-bound 2,4-D with free antibody provided significantly higher kinetic parameters (kinetic association rate constant) when using optically smooth crystals compared to common rough crystal. Therefore. the smooth crystal should be preferred for future kinetic studies. The competitive assay of the herbicide 2,4-D achieved a limit of detection of 10 ng/l using the monoclonal anti-2,4-D antibody F6C10. Finally, a direct assay format has been evaluated using a thicker layer of glutaraldehyde-crosslinked antibody on the sensing surface. The direct binding of a small herbicide molecule was follon ed in real time. The detected concentration of 2,4-D (5 ug/l) was low enough for future direct monitoring of this herbicide in water.
OC 518.30, research and development projectName: Sledování interakcí biomakromolekul s biovrstvami v různých konformačních stavech pomocí piezoelektrických biosensorů
Investor: Ministry of Education, Youth and Sports of the CR, Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors
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