Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a
Vacuum Deposition Interface

J 2002

Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface

PREISLER, Jan, Ping HU, Barry, L. KARGER, Tomáš REJTAR, Eugene MOSKOVEETS et. al.

Základní údaje

Originální název

Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface

Autoři

PREISLER, Jan (203 Česká republika, garant), Ping HU (156 Čína), Barry, L. KARGER (840 Spojené státy), Tomáš REJTAR (203 Česká republika) a Eugene MOSKOVEETS (643 Rusko)

Vydání

Analytical Chemistry, Washington, D.C., USA, American Chemical Society, 2002, 0003-2700

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.094

Kód RIV

RIV/00216224:14310/02:00008542

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000173086200010

Klíčová slova anglicky

capillary array electrophoresis; MALDI; mass spectrometry; proteomics; peptide

Štítky

capillary array electrophoresis, MALDI, mass spectrometry, peptide, proteomics

Změněno: 28. 6. 2009 00:01, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

The approach of vacuum deposition has been extended to demonstrate parallel analysis for multiple on-line infusion MALDI TOF MS and capillary array electrophoresis (CAE)-MALDI MS. In the infusion mode, individual peptide samples were simultaneously deposited on a Mylar tape cartridge using an array of eight capillaries, yielding eight parallel traces. For CAE-MALDI/TOF MS, the same number of separation capillaries were coupled with an array of eight infusion capillaries using a common liquid junction, containing matrix solution. A fast-scanning mirror was employed to traverse the beam of the desorption laser across the Mylar tape to probe one trace at a time. The positions of the eight sample traces formed on the tape were automatically determined, and all samples were analyzed in rapid sequence using a kilohertz repetition rate laser and a high-throughput data acquisition system. The instrumentation was operated with CAE MS for high-throughput analysis without compromising data quality applicable to MALDI/TOF MS analysis for proteomics and other areas where separation and high throughput are required.

Citovat

PREISLER, Jan, Ping HU, Barry, L. KARGER, Tomáš REJTAR a Eugene MOSKOVEETS. Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface. Analytical Chemistry. Washington, D.C., USA: American Chemical Society, 2002, roč. 74, č. 1, s. 17-25. ISSN 0003-2700.

@article{384181,
   author = {Preisler, Jan and Hu, Ping and Karger, Barry, L. and Rejtar, Tomáš and Moskoveets, Eugene},
   article_location = {Washington, D.C., USA},
   article_number = {1},
   keywords = {capillary array electrophoresis; MALDI; mass spectrometry; proteomics; peptide},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical Chemistry},
   title = {Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface},
   volume = {74},
   year = {2002}
}

TY  - JOUR
ID  - 384181
AU  - Preisler, Jan - Hu, Ping - Karger, Barry, L. - Rejtar, Tomáš - Moskoveets, Eugene
PY  - 2002
TI  - Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface
JF  - Analytical Chemistry
VL  - 74
IS  - 1
SP  - 17
EP  - 17
PB  - American Chemical Society
SN  - 00032700
KW  - capillary array electrophoresis
KW  - MALDI
KW  - mass spectrometry
KW  - proteomics
KW  - peptide
N2  - The approach of vacuum deposition has been extended to demonstrate parallel analysis for multiple on-line infusion MALDI TOF MS and capillary array electrophoresis (CAE)-MALDI MS. In the infusion mode, individual peptide samples were simultaneously deposited on a Mylar tape cartridge using an array of eight capillaries, yielding eight parallel traces. For CAE-MALDI/TOF MS, the same number of separation capillaries were coupled with an array of eight infusion capillaries using a common liquid junction, containing matrix solution. A fast-scanning mirror was employed to traverse the beam of the desorption laser across the Mylar tape to probe one trace at a time. The positions of the eight sample traces formed on the tape were automatically determined, and all samples were analyzed in rapid sequence using a kilohertz repetition rate laser and a high-throughput data acquisition system. The instrumentation was operated with CAE MS for high-throughput analysis without compromising data quality applicable to MALDI/TOF MS analysis for proteomics and other areas where separation and high throughput are required.
ER  -

PREISLER, Jan, Ping HU, Barry, L. KARGER, Tomáš REJTAR a Eugene MOSKOVEETS. Capillary Array Electrophoresis-MALDI Mass Spectrometry Using a Vacuum Deposition Interface. \textit{Analytical Chemistry}. Washington, D.C., USA: American Chemical Society, 2002, roč.~74, č.~1, s.~17-25. ISSN~0003-2700.

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