Cyclooxygenase and Lipoxygenase Inhibitors Potentiate
Antiproliferative, Apoptotic and Differentiation effects of
TNFa on human leukemic HL-60 cells

D 2000

Cyclooxygenase and Lipoxygenase Inhibitors Potentiate Antiproliferative, Apoptotic and Differentiation effects of TNFa on human leukemic HL-60 cells

ŠTIKA, Jiří, Jan VONDRÁČEK, Jiřina HOFMANOVÁ, Vladimír ŠIMEK, Alois KOZUBÍK et. al.

Basic information

Original name

Cyclooxygenase and Lipoxygenase Inhibitors Potentiate Antiproliferative, Apoptotic and Differentiation effects of TNFa on human leukemic HL-60 cells

Authors

ŠTIKA, Jiří, Jan VONDRÁČEK, Jiřina HOFMANOVÁ, Vladimír ŠIMEK and Alois KOZUBÍK

Edition

Berlín, SRN, 11th European Students' Conference at the Charité Berlin, p. 141-141, 2000

Publisher

Charité

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30105 Physiology

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/00:00005237

Organization unit

Faculty of Science

Změněno: 8/1/2002 15:58, Mgr. Jiří Štika, Ph.D.

Abstract

V originále

Our results indicated that indomethacin alone has only minor effects on the measured parameters, however it potentiated (in concentration dependent manner) antiproliferative and apoptotic effects of TNFa, as well as CD11b/14 expression. NSE activity and ROI production after TNFa treatment was not influenced by indomethacin. After combined treatment of cells with indomethacin and TNFa an increased percentage of cells in S-phase of the cell cycle was observed. On the other hand, MK-886 alone dose-dependently reduced proliferation and viability of HL-60 cells, induced apoptosis and increased amount of cells in G1 phase of the cell cycle. These effects were further potentiated by combination with TNFa. Moreover, when combined with TNFa MK-886 significantly increased NSE activity, ROI production and CD11b/14 expression, i. e. it potentiated differentiation of HL-60 cells. In conclusion, while inhibition of cyclooxygenase potentiates the antiproliferative and the apoptotic effects of TNFa, inhibition of lipoxygenase significantly increases also the differentiation induced by TNFa. These results may be important for searching new strategies of antileukemic therapy.

Links

GA524/99/0694, research and development project

Name: Vysoce nenasycené mastné kyseliny a cytokiny - jejich úloha pro zachování homeostázy na úrovni buněčných populací

Citovat

ŠTIKA, Jiří, Jan VONDRÁČEK, Jiřina HOFMANOVÁ, Vladimír ŠIMEK and Alois KOZUBÍK. Cyclooxygenase and Lipoxygenase Inhibitors Potentiate Antiproliferative, Apoptotic and Differentiation effects of TNFa on human leukemic HL-60 cells. In 11th European Students' Conference at the Charité Berlin. Berlín, SRN: Charité, 2000, p. 141.

@inproceedings{387174,
   author = {Štika, Jiří and Vondráček, Jan and Hofmanová, Jiřina and Šimek, Vladimír and Kozubík, Alois},
   address = {Berlín, SRN},
   booktitle = {11th European Students' Conference at the Charité Berlin},
   language = {eng},
   location = {Berlín, SRN},
   pages = {141-141},
   publisher = {Charité},
   title = {Cyclooxygenase and Lipoxygenase Inhibitors Potentiate Antiproliferative, Apoptotic and Differentiation effects of TNFa on human leukemic HL-60 cells},
   year = {2000}
}

TY  - JOUR
ID  - 387174
AU  - Štika, Jiří - Vondráček, Jan - Hofmanová, Jiřina - Šimek, Vladimír - Kozubík, Alois
PY  - 2000
TI  - Cyclooxygenase and Lipoxygenase Inhibitors Potentiate Antiproliferative, Apoptotic and Differentiation effects of TNFa on human leukemic HL-60 cells
PB  - Charité
CY  - Berlín, SRN
N2  - Our results indicated that indomethacin alone has only minor effects on the measured parameters, however it potentiated (in concentration dependent manner) antiproliferative and apoptotic effects of TNFa, as well as CD11b/14 expression. NSE activity and ROI production after TNFa treatment was not influenced by indomethacin. After combined treatment of cells with indomethacin and TNFa an increased percentage of cells in S-phase of the cell cycle was observed. On the other hand, MK-886 alone dose-dependently reduced proliferation and viability of HL-60 cells, induced apoptosis and increased amount of cells in G1 phase of the cell cycle. These effects were further potentiated by combination with TNFa. Moreover, when combined with TNFa MK-886 significantly increased NSE activity, ROI production and CD11b/14 expression, i. e. it potentiated differentiation of HL-60 cells. In conclusion, while inhibition of cyclooxygenase potentiates the antiproliferative and the apoptotic effects of TNFa, inhibition of lipoxygenase significantly increases also the differentiation induced by TNFa. These results may be important for searching new strategies of antileukemic therapy.
ER  -

ŠTIKA, Jiří, Jan VONDRÁČEK, Jiřina HOFMANOVÁ, Vladimír ŠIMEK and Alois KOZUBÍK. Cyclooxygenase and Lipoxygenase Inhibitors Potentiate Antiproliferative, Apoptotic and Differentiation effects of TNFa on human leukemic HL-60 cells. In \textit{11th European Students' Conference at the Charité Berlin}. Berlín, SRN: Charité, 2000, p.~141.

Detailed Information on Publication Record