Immunomicroscopic studies on the neuromusculature of the
diplozoid monogenean, Eudiplozoon nipponicum, during fusion and
maturation.

D 2001

Immunomicroscopic studies on the neuromusculature of the diplozoid monogenean, Eudiplozoon nipponicum, during fusion and maturation.

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, G. BRENNAN, A. MAULE et. al.

Základní údaje

Originální název

Immunomicroscopic studies on the neuromusculature of the diplozoid monogenean, Eudiplozoon nipponicum, during fusion and maturation.

Autoři

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, G. BRENNAN, A. MAULE, Milan GELNAR a D. HALTON

Vydání

2001. vyd. Brisbane, Australie. 4th International Symposium on Monogenea. Abstract Book. s. 75-75, 2001

Nakladatel

The University of Queensland, Brisbane, Queensland, Australia.

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Austrálie

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/01:00005365

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

monogenea; neuromascular system; confocal microscopy

Štítky

confocal microscopy, Monogenea, neuromascular system

Změněno: 18. 1. 2002 15:38, Mgr. Tomáš Zurawski, Ph.D.

Anotace

V originále

Diplozoidae monogeneans are unique fish-gill parasites comprising two individuals fused in permanent copula. Fusion begins when two larvae (diporpae) meet on the host gill, prompting maturation to the adult worm. We have examined microscopically the neuromusculature of Eudiplozoon nipponicum to explore neuromuscular involvement in fusion. Cholinergic, aminergic and peptidergic neuronal elements were demonstrated by enzyme- and immuno-cytochemistry in conjunction with confocal microscopy. FITC-labelled phalloidin was a probe for F-actin. Electron immunogold-labeling revealed peptide immunoreactivity subcellularly. The nervous system was strongly reactive for co-localised cholinergic and peptidergic pathways and for separate pathways of aminergic elements. There was strong innervation to the pharynx, attachment sucker and clamps of the diporpa. Neuronal pathways of worm pairs are separate, with no obvious fusion. Muscle systems are dominated by extensive somatic fibres, buccal suckers, haptoral clamps and, in early stages, a transient ventral sucker. Diagonal fibres largely comprise the diporpa body wall and longitudinal muscle bundles run from forebody to haptor. EM confirmed neuropeptide-immunolocalisation in dense-cored vesicles in axons of both CNS and PNS. The structural complexity of the neuromusculature reflects its importance in host attachment, and in the processes of body contact, twisting/fusion in larval pairing.

Návaznosti

GA524/00/0844, projekt VaV

Název: Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

Investor: Grantová agentura ČR, Neurobiologie, stavba tegumentu a segregace mikrohabitu modelových druhů cizopasníků v průběhu ontogeneze

MSM 143100010, záměr

Název: Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy.

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časoprostorová dynamika biodiverzity v ekosystémech střední Evropy

Citovat

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, G. BRENNAN, A. MAULE, Milan GELNAR a D. HALTON. Immunomicroscopic studies on the neuromusculature of the diplozoid monogenean, Eudiplozoon nipponicum, during fusion and maturation. In 4th International Symposium on Monogenea. Abstract Book. 2001. vyd. Brisbane, Australie.: The University of Queensland, Brisbane, Queensland, Australia., 2001, s. 75.

@inproceedings{390056,
   author = {Zurawski, Tomáš and Mousley, A. and Mair, G. and Brennan, G. and Maule, A. and Gelnar, Milan and Halton, D.},
   address = {Brisbane, Australie.},
   booktitle = {4th International Symposium on Monogenea. Abstract Book.},
   edition = {2001},
   keywords = {monogenea; neuromascular system; confocal microscopy},
   language = {eng},
   location = {Brisbane, Australie.},
   pages = {75-75},
   publisher = {The University of Queensland, Brisbane, Queensland, Australia.},
   title = {Immunomicroscopic studies on the neuromusculature of the diplozoid monogenean, Eudiplozoon nipponicum, during fusion and maturation.},
   year = {2001}
}

TY  - JOUR
ID  - 390056
AU  - Zurawski, Tomáš - Mousley, A. - Mair, G. - Brennan, G. - Maule, A. - Gelnar, Milan - Halton, D.
PY  - 2001
TI  - Immunomicroscopic studies on the neuromusculature of the diplozoid monogenean, Eudiplozoon nipponicum, during fusion and maturation.
PB  - The University of Queensland, Brisbane, Queensland, Australia.
CY  - Brisbane, Australie.
KW  - monogenea
KW  - neuromascular system
KW  - confocal microscopy
N2  - Diplozoidae monogeneans are unique fish-gill parasites comprising two individuals fused in permanent copula. Fusion begins when two larvae (diporpae) meet on the host gill, prompting maturation to the adult worm. We have examined microscopically the neuromusculature of Eudiplozoon nipponicum to explore neuromuscular involvement in fusion. Cholinergic, aminergic and peptidergic neuronal elements were demonstrated by enzyme- and immuno-cytochemistry in conjunction with confocal microscopy. FITC-labelled phalloidin was a probe for F-actin. Electron immunogold-labeling revealed peptide immunoreactivity subcellularly. The nervous system was strongly reactive for co-localised cholinergic and peptidergic pathways and for separate pathways of aminergic elements. There was strong innervation to the pharynx, attachment sucker and clamps of the diporpa. Neuronal pathways of worm pairs are separate, with no obvious fusion. Muscle systems are dominated by extensive somatic fibres, buccal suckers, haptoral clamps and, in early stages, a transient ventral sucker. Diagonal fibres largely comprise the diporpa body wall and longitudinal muscle bundles run from forebody to haptor. EM confirmed neuropeptide-immunolocalisation in dense-cored vesicles in axons of both CNS and PNS. The structural complexity of the neuromusculature reflects its importance in host attachment, and in the processes of body contact, twisting/fusion in larval pairing.
ER  -

ZURAWSKI, Tomáš, A. MOUSLEY, G. MAIR, G. BRENNAN, A. MAULE, Milan GELNAR a D. HALTON. Immunomicroscopic studies on the neuromusculature of the diplozoid monogenean, Eudiplozoon nipponicum, during fusion and maturation. In \textit{4th International Symposium on Monogenea. Abstract Book.}. 2001. vyd. Brisbane, Australie.: The University of Queensland, Brisbane, Queensland, Australia., 2001, s.~75.

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