Elimination Voltammetry of Adenine and Cytosine  Mixtures

J 2001

Elimination Voltammetry of Adenine and Cytosine Mixtures

TRNKOVÁ, Libuše; Jiří FRIML and Oldřich DRAČKA

Basic information

Original name

Elimination Voltammetry of Adenine and Cytosine Mixtures

Authors

TRNKOVÁ, Libuše; Jiří FRIML and Oldřich DRAČKA

Edition

Bioelectrochemistry, The Netherlands, Elsevier, 2001, 1567-5394

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10405 Electrochemistry

Country of publisher

Czech Republic

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 1.096

RIV identification code

RIV/00216224:14310/01:00005416

Organization unit

Faculty of Science

Keywords in English

Elimination voltammetry with linear scan; cytosine; adenine; reduction of bases; hydrogen evolution

Abstract

In the original language

The elimination voltammetry with linear scan (EVLS) was used to study adenine and cytosine reduction signals at the mercury electrode. In comparison with the linear scan voltammetry providing only one unresolved peak, two elimination functions provide good resolution of individual peaks and significant increase of sensitivity.Two selected functions give two well resolved peaks of adenine and cytosine in a wide concentration range, while the linear sweep voltammetry gives badly resolved peaks due to the hydrogen evolution. The best resolution of peaks is observed in acetate buffer at pH 3.8 and a detection limit for both substances is 500 nM. The concentration dependence of EVLS peak heights for one substance at the constant concentration of the other substance is linear. The peak potentials differ in these elimination functions. The difference in EVLS peak potentials gives the possibility to evaluate .EVLS contributes to the resolution of cathodic signals of purine and pyrimidine bases at very negative potentials near supporting electrolyte discharge.

Links

GV204/97/K084, research and development project

Name: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu

Investor: Czech Science Foundation, Electrodes modified with nucleic acids and proteins. New tools in biochemical and biomedical research

MSM 143100011, plan (intention)

Name: Struktura a vazebné poměry, vlastnosti a analýza syntetických a přírodních molekulových ansamblů

Investor: Ministry of Education, Youth and Sports of the CR, Structure and character of bonding, properties and analysis of synthetic and natural molecular ensembles

Cite

TRNKOVÁ, Libuše; Jiří FRIML and Oldřich DRAČKA. Elimination Voltammetry of Adenine and Cytosine Mixtures. Bioelectrochemistry. The Netherlands: Elsevier, 2001, vol. 2001, No 54, p. 131-136. ISSN 1567-5394.

@article{390892,
   author = {Trnková, Libuše and Friml, Jiří and Dračka, Oldřich},
   article_location = {The Netherlands},
   article_number = {54},
   keywords = {Elimination voltammetry with linear scan; cytosine; adenine; reduction of bases; hydrogen evolution},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Elimination Voltammetry of Adenine and Cytosine Mixtures},
   volume = {2001},
   year = {2001}
}

TY  - JOUR
ID  - 390892
AU  - Trnková, Libuše - Friml, Jiří - Dračka, Oldřich
PY  - 2001
TI  - Elimination Voltammetry of Adenine and Cytosine Mixtures
JF  - Bioelectrochemistry
VL  - 2001
IS  - 54
SP  - 131
EP  - 131
PB  - Elsevier
SN  - 15675394
KW  - Elimination voltammetry with linear scan
KW  - cytosine
KW  - adenine
KW  - reduction of bases
KW  - hydrogen evolution
N2  - The elimination voltammetry with linear scan (EVLS) was used to study adenine and cytosine reduction signals at the mercury electrode. In comparison with the linear scan voltammetry providing only one unresolved peak, two elimination functions provide good resolution of individual peaks and significant increase of sensitivity.Two selected functions give two well resolved peaks of adenine and cytosine in a wide concentration range, while the linear sweep voltammetry gives badly resolved peaks due to the hydrogen evolution. The best resolution of peaks is observed in acetate buffer at pH 3.8 and a detection limit for both substances is 500 nM. The concentration dependence of EVLS peak heights for one substance at the constant concentration of the other substance is linear. The peak potentials differ in these elimination functions. The difference in EVLS peak potentials gives the possibility to evaluate .EVLS contributes to the resolution of cathodic signals of purine and pyrimidine bases at very negative potentials near supporting electrolyte discharge.
ER  -

TRNKOVÁ, Libuše; Jiří FRIML and Oldřich DRAČKA. Elimination Voltammetry of Adenine and Cytosine Mixtures. \textit{Bioelectrochemistry}. The Netherlands: Elsevier, 2001, vol.~2001, No~54, p.~131-136. ISSN~1567-5394.

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