Advances in high-resolution cytometry of FISH dots in
interphase cell nuclei

a 2000

Advances in high-resolution cytometry of FISH dots in interphase cell nuclei

KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ et. al.

Základní údaje

Originální název

Advances in high-resolution cytometry of FISH dots in interphase cell nuclei

Autoři

KOZUBEK, Michal (203 Česká republika, garant), Stanislav KOZUBEK (203 Česká republika), Emilie LUKÁŠOVÁ (203 Česká republika), Eva BÁRTOVÁ (203 Česká republika), Magdalena SKALNÍKOVÁ (203 Česká republika), Pavel MATULA (203 Česká republika), Petr MATULA (203 Česká republika), Pavla JIRSOVÁ (203 Česká republika), Alena GAŇOVÁ (203 Česká republika) a Irena KOUTNÁ (203 Česká republika)

Vydání

The XX Congress of the International Society for Analytical Cytology, 2000

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14330/00:00005455

Organizační jednotka

Fakulta informatiky

Klíčová slova anglicky

automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis

Štítky

3-D analysis, automated microscopy, fluorescence in situ hybridization, high-resolution cytometry, Image analysis, interphase nuclei

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 7. 5. 2010 16:58, prof. RNDr. Michal Kozubek, Ph.D.

Anotace

V originále

Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.

Návaznosti

MSM 143300002, záměr

Název: Využití počítačové analýzy obrazu v optické mikroskopii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Využití počítačové analýzy obrazu v optické mikroskopii

Citovat

KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavel MATULA, Petr MATULA, Pavla JIRSOVÁ, Alena GAŇOVÁ a Irena KOUTNÁ. Advances in high-resolution cytometry of FISH dots in interphase cell nuclei. In The XX Congress of the International Society for Analytical Cytology. 2000.

@proceedings{391753,
   author = {Kozubek, Michal and Kozubek, Stanislav and Lukášová, Emilie and Bártová, Eva and Skalníková, Magdalena and Matula, Pavel and Matula, Petr and Jirsová, Pavla and Gaňová, Alena and Koutná, Irena},
   booktitle = {The XX Congress of the International Society for Analytical Cytology},
   keywords = {automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis},
   language = {eng},
   title = {Advances in high-resolution cytometry of FISH dots in interphase cell nuclei},
   year = {2000}
}

TY  - CONF
ID  - 391753
AU  - Kozubek, Michal - Kozubek, Stanislav - Lukášová, Emilie - Bártová, Eva - Skalníková, Magdalena - Matula, Pavel - Matula, Petr - Jirsová, Pavla - Gaňová, Alena - Koutná, Irena
PY  - 2000
TI  - Advances in high-resolution cytometry of FISH dots in interphase cell nuclei
KW  - automated microscopy
KW  - image analysis
KW  - high-resolution cytometry
KW  - fluorescence in situ hybridization
KW  - interphase nuclei
KW  - 3-D analysis
N2  - Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies.
ER  -

KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavel MATULA, Petr MATULA, Pavla JIRSOVÁ, Alena GAŇOVÁ a Irena KOUTNÁ. Advances in high-resolution cytometry of FISH dots in interphase cell nuclei. In \textit{The XX Congress of the International Society for Analytical Cytology}. 2000.

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