LUKÁŠOVÁ, Emilie, Stanislav KOZUBEK, Michal KOZUBEK, Václav KROHA, Andrea MAREČKOVÁ, Magdalena SKALNÍKOVÁ and Eva BÁRTOVÁ. Chromosomes participating in translocations typical of malignant haemoblastoses are also involved in frequent interchanges induced by densely ionising radiation. In The XX Congress of the International Society for Analytical Cytology. 2000.
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Original name Chromosomes participating in translocations typical of malignant haemoblastoses are also involved in frequent interchanges induced by densely ionising radiation
Authors LUKÁŠOVÁ, Emilie (203 Czech Republic), Stanislav KOZUBEK (203 Czech Republic), Michal KOZUBEK (203 Czech Republic), Václav KROHA, Andrea MAREČKOVÁ, Magdalena SKALNÍKOVÁ (203 Czech Republic) and Eva BÁRTOVÁ (203 Czech Republic).
Edition The XX Congress of the International Society for Analytical Cytology, 2000.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10610 Biophysics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14330/00:00005456
Organization unit Faculty of Informatics
Keywords in English exchange aberrations; repeated FISH; chromosome arrangement; interphase nucleus; human lymphocytes
Tags chromosome arrangement, exchange aberrations, human lymphocytes, interphase nucleus, repeated FISH
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Michal Kozubek, Ph.D., učo 3740. Changed: 7/5/2010 17:01.
Abstract
Repeated triple-colour fluorescence in situ hybridisation was used for the detection of exchange aberrations among 10 selected chromosomes of human lymphocytes irradiated with fast neutrons and He ions of mean energy of 7 MeV and 30 MeV respectively. In each hybridisation two different pairs of chromosomes were stained. Defined stage positions of metaphases on a slide were stored on a hard disk and an automatic scan of images according to these positions was performed after six successive hybridisations. In this way, six different images of the same metaphase with differently stained pairs of chromosomes and all centromeres were obtained.
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MSM 143300002, plan (intention)Name: Využití počítačové analýzy obrazu v optické mikroskopii
Investor: Ministry of Education, Youth and Sports of the CR, Application of computer image analysis in optical microscopy
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