Other formats:
BibTeX
LaTeX
RIS
@proceedings{391792, author = {Kozubek, Michal and Kozubek, Stanislav and Lukášová, Emilie and Bártová, Eva and Skalníková, Magdalena and Matula, Pavel and Matula, Petr and Jirsová, Pavla and Gaňová, Alena and Koutná, Irena}, booktitle = {Cytokinematics 2000}, keywords = {automated microscopy; image analysis; high-resolution cytometry; fluorescence in situ hybridization; interphase nuclei; 3-D analysis}, language = {eng}, title = {Advances in high-resolution cytometry of FISH dots in interphase cell nuclei}, year = {2000} }
TY - CONF ID - 391792 AU - Kozubek, Michal - Kozubek, Stanislav - Lukášová, Emilie - Bártová, Eva - Skalníková, Magdalena - Matula, Pavel - Matula, Petr - Jirsová, Pavla - Gaňová, Alena - Koutná, Irena PY - 2000 TI - Advances in high-resolution cytometry of FISH dots in interphase cell nuclei KW - automated microscopy KW - image analysis KW - high-resolution cytometry KW - fluorescence in situ hybridization KW - interphase nuclei KW - 3-D analysis N2 - Recently, we have described a high-resolution cytometry (HRCM) technique (Cytometry 36:279-293) as a reasonable compromise between flow cytometry (FCM) and laser scanning cytometry (LSCM) on one hand and confocal laser scanning microscopy (CLSM) on the other hand. Whereas FCM/LSCM and CLSM enable measuring of either large number of cells with low resolution or small number of cells with high resolution, respectively, HRCM can analyze quantities comparable to FCM/LSCM measurements (using overnight acquisition) with an accuracy comparable to CLSM. Very recently we have built a second HRCM instrument (see picture at http://www.fi.muni.cz/lom) which enables switching between conventional and confocal modes. The confocal mode is realized using a 5% Nipkow disk optimized for fluorescence imaging. It offers a better resolution but at a lower speed. The user can choose which fluorochromes are imaged in confocal mode. Thus, the system can be configured for a variety of imaging modes ranging from quick 2-D analysis up to high-resolution confocal 3-D studies. ER -
KOZUBEK, Michal, Stanislav KOZUBEK, Emilie LUKÁŠOVÁ, Eva BÁRTOVÁ, Magdalena SKALNÍKOVÁ, Pavel MATULA, Petr MATULA, Pavla JIRSOVÁ, Alena GAŇOVÁ and Irena KOUTNÁ. Advances in high-resolution cytometry of FISH dots in interphase cell nuclei. In \textit{Cytokinematics 2000}. 2000.
|