Další formáty:
BibTeX
LaTeX
RIS
@article{397051, author = {Rejtar, Tomas and Ping, Hu and Juhasz, Peter and Campbell, Jennifer M. and Vestal, Marvin L. and Preisler, Jan and Karger, Barry, L.}, article_location = {Washington, USA}, article_number = {2}, keywords = {MALDI; mass spectrometry; capillary electrophoresis; proteomics; proteins; peptides; interface;}, language = {eng}, issn = {1535-3893}, journal = {Journal of Proteome Research}, title = {Off-Line Coupling of High-Resolution Capillary Electrophoresis to MALDI-TOF and TOF/TOF MS}, volume = {1}, year = {2002} }
TY - JOUR ID - 397051 AU - Rejtar, Tomas - Ping, Hu - Juhasz, Peter - Campbell, Jennifer M. - Vestal, Marvin L. - Preisler, Jan - Karger, Barry, L. PY - 2002 TI - Off-Line Coupling of High-Resolution Capillary Electrophoresis to MALDI-TOF and TOF/TOF MS JF - Journal of Proteome Research VL - 1 IS - 2 SP - 171 EP - 171 PB - ACS SN - 15353893 KW - MALDI KW - mass spectrometry KW - capillary electrophoresis KW - proteomics KW - proteins KW - peptides KW - interface; N2 - High-resolution capillary electrophoresis has been coupled to MALDI-TOF and TOF/TOF MS through off-line vacuum deposition onto standard stainless steel MALDI targets. This off-line approach allowed the decoupling of the separation from the MS analysis, thus allowing each to be independently optimized in terms of time. Using BSA tryptic digest as a model sample, the deposited streaks, roughly 100-ím wide, were first analyzed in the MS mode, consuming only a fraction of the sample. After data analysis, segments of the deposited trace, containing unidentified peptides, as well as several species chosen for sequence confirmation, were reanalyzed in the MS/MS mode using MALDI-TOF/TOF MS. Additionally, it is shown that the shot-to-shot reproducibility of the vacuum-deposited trace (5% RSD) is 1 order of magnitude lower than that found for the standard dried droplet method. Moreover, a linear dependence of signal intensities (relative to an internal standard) over 3 orders of magnitude was found for a peptide sample with concentrations ranging from 1 to 1000 nM. This paper demonstrates the potential of off-line coupling of high-resolution separations to MALDI-MS and MALDI-MS/MS using vacuum deposition for the analysis of complex peptide mixtures from protein digests. ER -
REJTAR, Tomas, Hu PING, Peter JUHASZ, Jennifer M. CAMPBELL, Marvin L. VESTAL, Jan PREISLER a Barry, L. KARGER. Off-Line Coupling of High-Resolution Capillary Electrophoresis to MALDI-TOF and TOF/TOF MS. \textit{Journal of Proteome Research}. Washington, USA: ACS, 2002, roč.~1, č.~2, s.~171-179. ISSN~1535-3893.
|