Residual expression of functional MHC class II molecules in
twin brothers with MHC class II deficiency is cell type
specific

J 2001

Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific

WOLF, HM., Vojtěch THON, S. LECHLEITNER, Marta EIBL, P. PETZELBAUER et. al.

Základní údaje

Originální název

Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific

Název česky

Zbytková exprese funkčních molekul MHC II. třídy u dvojčat je buněčně specifická

Autoři

WOLF, HM. (40 Rakousko), Vojtěch THON (203 Česká republika, garant), S. LECHLEITNER (40 Rakousko), Marta EIBL (40 Rakousko) a P. PETZELBAUER (40 Rakousko)

Vydání

British Journal of Haematology, 2001, 0007-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.815

Kód RIV

RIV/00216224:14110/01:00030461

Organizační jednotka

Lékařská fakulta

UT WoS

000172403300036

Klíčová slova česky

deficience MHC molekul II. třídy; dendritické buňky

Klíčová slova anglicky

MHC class II deficiency; dendritic cells; endothelial cells; antigen presentation; residual cell type-specific MHC class II expression

Štítky

antigen presentation, dendritic cells, endothelial cells, MHC class II deficiency

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 6. 2009 00:13, prof. MUDr. Vojtěch Thon, Ph.D.

Anotace

ORIG CZ

V originále

We examined major histocompatibility complex (MHC) class II expression in B cells, peripheral blood monocytes, activated T cells, epidermal Langerhans cells, monocyte-derived dendritic cells, dermal microvascular endothelial cells (DMEC) and fibroblasts of twin brothers with MHC class II deficiency. Although residual human leucocyte antigen (HLA)-DR expression was found on a subpopulation of epidermal Langerhans cells and a subset of peripheral blood monocyte-derived dendritic cells, the patients' B cells, monocytes and activated T cells were HLA-DR negative. After treatment with interferon-gamma (IFN-gamma), the patients' DMEC expressed HLA-DR but not -DP and -DQ at the protein and mRNA level, whereas IFN-gamma failed to induce HLA-DR expression on dermal fibroblasts. The patients' monocyte-derived dendritic cells were capable of processing and presenting tetanus toxoid to autologous T cells, and patient-derived DMEC induced the proliferation of allogeneic CD4(+) T cells in an MHC class II-restricted fashion, indicating that the observed residual MHC class II surface expression was functional. The findings reported show that the defect encountered in these patients is not necessarily expressed to the same extent in different cell lineages, which is relevant for the understanding of the patients' phenotype and also illustrates that only small amounts of MHC class II are needed to mount a functional cellular immune response in vivo.

Česky

Zbytková exprese funkčních molekul MHC II. třídy u dvojčat je buněčně specifická.

Citovat

WOLF, HM., Vojtěch THON, S. LECHLEITNER, Marta EIBL a P. PETZELBAUER. Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific. British Journal of Haematology. 2001, roč. 115, č. 2, s. 460-471. ISSN 0007-1048.

@article{397101,
   author = {Wolf, HM. and Thon, Vojtěch and Lechleitner, S. and Eibl, Marta and Petzelbauer, P.},
   article_number = {2},
   keywords = {MHC class II deficiency; dendritic cells; endothelial cells; antigen presentation; residual cell type-specific MHC class II expression},
   language = {eng},
   issn = {0007-1048},
   journal = {British Journal of Haematology},
   title = {Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific},
   volume = {115},
   year = {2001}
}

TY  - JOUR
ID  - 397101
AU  - Wolf, HM. - Thon, Vojtěch - Lechleitner, S. - Eibl, Marta - Petzelbauer, P.
PY  - 2001
TI  - Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific
JF  - British Journal of Haematology
VL  - 115
IS  - 2
SP  - 460
EP  - 460
SN  - 00071048
KW  - MHC class II deficiency
KW  - dendritic cells
KW  - endothelial cells
KW  - antigen presentation
KW  - residual cell type-specific MHC class II expression
N2  - We examined major histocompatibility complex (MHC) class II expression in B cells, peripheral blood monocytes, activated T cells, epidermal Langerhans cells, monocyte-derived dendritic cells, dermal microvascular endothelial cells (DMEC) and fibroblasts of twin brothers with MHC class II deficiency. Although residual human leucocyte antigen (HLA)-DR expression was found on a subpopulation of epidermal Langerhans cells and a subset of peripheral blood monocyte-derived dendritic cells, the patients' B cells, monocytes and activated T cells were HLA-DR negative. After treatment with interferon-gamma (IFN-gamma), the patients' DMEC expressed HLA-DR but not -DP and -DQ at the protein and mRNA level, whereas IFN-gamma failed to induce HLA-DR expression on dermal fibroblasts. The patients' monocyte-derived dendritic cells were capable of processing and presenting tetanus toxoid to autologous T cells, and patient-derived DMEC induced the proliferation of allogeneic CD4(+) T cells in an MHC class II-restricted fashion, indicating that the observed residual MHC class II surface expression was functional. The findings reported show that the defect encountered in these patients is not necessarily expressed to the same extent in different cell lineages, which is relevant for the understanding of the patients' phenotype and also illustrates that only small amounts of MHC class II are needed to mount a functional cellular immune response in vivo.
ER  -

WOLF, HM., Vojtěch THON, S. LECHLEITNER, Marta EIBL a P. PETZELBAUER. Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific. \textit{British Journal of Haematology}. 2001, roč.~115, č.~2, s.~460-471. ISSN~0007-1048.

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