Double mutant and formaldehyde inactivated TSST-1 as vaccine
candidates

J 2002

Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates

GAMPFER, J., Vojtěch THON, H. GULLE, Hermann WOLF, Marta EIBL et. al.

Základní údaje

Originální název

Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates

Název česky

Inaktivované TSST-1 jako kandidátní vakcíny pro syndrom tox. šoku

Autoři

GAMPFER, J. (276 Německo), Vojtěch THON (203 Česká republika, garant), H. GULLE (40 Rakousko), Hermann WOLF (40 Rakousko) a Marta EIBL (40 Rakousko)

Vydání

Vaccine, 2002, 0264-410X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.811

Kód RIV

RIV/00216224:14110/02:00030462

Organizační jednotka

Lékařská fakulta

UT WoS

000173858300010

Klíčová slova česky

TSST-1 vakcína

Klíčová slova anglicky

TSST-1 vaccine

Štítky

TSST-1 vaccine

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 6. 2009 00:11, prof. MUDr. Vojtěch Thon, Ph.D.

Anotace

ORIG CZ

V originále

Up to now there is no treatment for staphylococcal toxic shock syndrome, a disease mainly induced by toxic shock syndrome toxin-1(TSST-1). There is great demand in finding means to control the disease, one of them is the development of an effective and safe vaccine against TSST-1. In this study we constructed a series of vaccine candidates and investigated their biological activity, toxicity, and potential to invoke an immune response. TSST-1 was isolated from Stahylococcus aureus supernatants and recombinantly expressed as a N-terminal 6x histidine-tagged protein in Escherichia coli. In order to obtain molecules with minimal toxicity we constructed single mutants (G31R and H135A) and one double mutant (G31R/H135A) with both residues exchanged. We also detoxified native TSST-1 isolated from S. aureus, and recombinantly expressed TSST-1 by treatment with formaldehyde. Functional activity of native and recombinant TSST-1 and grade of inocuity of mutants and toxoids was determined by investigating mitogenity, T-cell activation, and cytokine release upon stimulation of human mononuclear cells with the vaccine candidates. All substances were tested in a rabbit immunization study. After primary immunization and three additional boosts all vaccinated animals developed antibody titers against TSST-1 and were protected against challenge with a lethal doses of superantigen potentiated with lipopolysaccharide.

Česky

Inaktivované TSST-1 jako kandidátní vakcíny pro syndrom toxického šoku.

Citovat

GAMPFER, J., Vojtěch THON, H. GULLE, Hermann WOLF a Marta EIBL. Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates. Vaccine. 2002, roč. 20, 9-10, s. 1354-64, 11 s. ISSN 0264-410X.

@article{397102,
   author = {Gampfer, J. and Thon, Vojtěch and Gulle, H. and Wolf, Hermann and Eibl, Marta},
   article_number = {9-10},
   keywords = {TSST-1 vaccine},
   language = {eng},
   issn = {0264-410X},
   journal = {Vaccine},
   title = {Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates},
   volume = {20},
   year = {2002}
}

TY  - JOUR
ID  - 397102
AU  - Gampfer, J. - Thon, Vojtěch - Gulle, H. - Wolf, Hermann - Eibl, Marta
PY  - 2002
TI  - Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates
JF  - Vaccine
VL  - 20
IS  - 9-10
SP  - 1354-64
EP  - 1354-64
SN  - 0264410X
KW  - TSST-1 vaccine
N2  - Up to now there is no treatment for staphylococcal toxic shock syndrome, a disease mainly induced by toxic shock syndrome toxin-1(TSST-1). There is great demand in finding means to control the disease, one of them is the development of an effective and safe vaccine against TSST-1. In this study we constructed a series of vaccine candidates and investigated their biological activity, toxicity, and potential to invoke an immune response. TSST-1 was isolated from Stahylococcus aureus supernatants and recombinantly expressed as a N-terminal 6x histidine-tagged protein in Escherichia coli. In order to obtain molecules with minimal toxicity we constructed single mutants (G31R and H135A) and one double mutant (G31R/H135A) with both residues exchanged. We also detoxified native TSST-1 isolated from S. aureus, and recombinantly expressed TSST-1 by treatment with formaldehyde. Functional activity of native and recombinant TSST-1 and grade of inocuity of mutants and toxoids was determined by investigating mitogenity, T-cell activation, and cytokine release upon stimulation of human mononuclear cells with the vaccine candidates. All substances were tested in a rabbit immunization study. After primary immunization and three additional boosts all vaccinated animals developed antibody titers against TSST-1 and were protected against challenge with a lethal doses of superantigen potentiated with lipopolysaccharide.
ER  -

GAMPFER, J., Vojtěch THON, H. GULLE, Hermann WOLF a Marta EIBL. Double mutant and formaldehyde inactivated TSST-1 as vaccine candidates. \textit{Vaccine}. 2002, roč.~20, 9-10, s.~1354-64, 11 s. ISSN~0264-410X.

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